大肠杆菌表达重组人白细胞介素-9的纯化及部分性质  

Purification and Some Characteristics of Recombinant Human Interleukin 9 Expressed in E.coli

作　　者：张津辉[1] 蒋中华[1] 陈惠鹏[1] 魏汉东[1] 邢桂春[1] 贺福初[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《中国生物化学与分子生物学报》1998年第5期562-566,共5页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：表达重组人白细胞介素－９（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｉｎｔｅｒｌｅｕｋｉｎ－９ｒｈＩＬ－９）的大肠杆菌经破碎、包涵体洗涤、裂解提取、凝胶过滤和离子交换色谱分离，得到了电泳纯的ｒｈＩＬ－９，回收率６６％，分子量与理论值相符，有刺激小鼠骨髓巨核系集落生成的活性．Recombinant human interleukin 9(rhIL 9) expressed in E.coli was isolated and purified by washing and solubilization of inclusion body,gel filtration chromatography and ion exchange chromatography.The final product displayed a single band with a corresponding purity of 99.2% in SDS PAGE stained by Coomassie brilliant blue R 250,and the total recovery of rhIL 9 was 66%.Molecular weight of rhIL 9 determined by SDS PAGE was 15.5kD.Biological activity analysis showed that rhIL 9 increased CFU Meg formation of normal mouse bone marrow cells.This study provided the basis for large scale preparation and further research of rhIL 9.

关 键 词：白细胞介素-9 色谱法 蛋白质纯化 

分 类 号：Q510.3[生物学—生物化学]