蟾蜍毒素对S180荷瘤小鼠肉瘤细胞Mg^(2+)-ATP酶、葡萄糖-6-磷酸酶活性的影响  

Effect of ethanol extract of toad venom on the activity of Mg^(2+)-ATPase and G-6-Pase in bearing-S180 tumors mice

作　　者：赵明芳[1] 王哲[2] 魏龙春 侯科佐[1] 刘云鹏[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,沈阳110001 [2]辽宁中医药大学病理科,沈阳110032 [3]辽河油田中心医院麻醉科,盘锦124010

出　　处：《实用药物与临床》2009年第6期385-387,共3页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省教育厅青年基金资助项目(05L544)

摘　　要：目的探讨醇溶性蟾蜍毒素对S180荷瘤小鼠肉瘤细胞Mg2+-ATP酶(Mg2+-ATPase)、葡萄糖-6-磷酸酶(G-6-Pase)活性的影响。方法乙醇溶解蟾蜍分泌物原浆,采用减压蒸馏等方法,进一步部分分离纯化,获得醇溶性的蟾蜍毒素混合物(Ethanol extract of toad venom,简称EET),将其作用于S180荷瘤小鼠模型,计算肿瘤抑制率,应用酶化学染色法观察S180肉瘤细胞酶活性的变化。结果蟾蜍毒素组和氟脲嘧啶组的肿瘤抑制率分别为35.6%和36.9%(P<0.05),细胞质膜标志酶Mg2+-ATPase、内质网标志酶G-6-Pase反应颗粒变小,数量减少,密度变低,提示酶活性明显下降。结论蟾蜍毒素可降低S180荷瘤小鼠肉瘤细胞质膜标志酶Mg2+-AT-Pase及内质网标志酶G-6-Pase的活性,抑制肿瘤细胞的生长。Objective To observe the effect of ethanol extract of toad venom on the activity of Mg2＋-ATPase and G-6-Pase in bearing-S180 tumors mice.Methods The models of bearing solid tumors were made by incubated S180 cell suspension to back.All 60 mice were distributed into 3 groups and injected 0.5mg/kg ethanol extract of toad venom or fluorouracil to abdominal cavity,respectively.The mice of normal control group were injected normal sodium to abdominal cavity.The inhibition rate of solid tumors in mice were calculated.Enzyme cytochemistry was used to detect the activity of Mg2＋-ATPase and G-6-Pase in bearing-S180 tumors mice.Results The response granules of Mg2＋-ATPase and G-6-Pase became smaller,the amount of the granules became fewer,the density was lower after the administration of ethanol extract of toad venom in bearing-S180 tumors mice.The inhibition rate of tumor were 35.6% and 36.9%（P〈0.05）.Conclusion Ethanol extract of toad venom can inhibit tumor cells′ growth by decreasing the activity of Mg2＋-ATPase and G-6-Pase.

关 键 词：肿瘤 蟾蜍毒素 S180肉瘤 Mg2+-ATPase 葡萄糖-6-磷酸酶 

分 类 号：R285.5[医药卫生—中药学] R273[医药卫生—中医学]