导入三叶草素基因(tfx)的快生型大豆根瘤菌竞争结瘤能力的研究  

STUDY ON NODULATION COMPETITIVENESS OF SINORHIZOBIUM FREDH BEEN TRANSFERED BY TRIFOLITOXIN GENES

作　　者：陈大松[1] 周俊初[1] 

机构地区：[1]华中农业大学农业部农业微生物重点开放性实验室,武汉430070

出　　处：《微生物学杂志》1998年第3期1-5,共5页Journal of Microbiology

摘　　要：以导入三叶草素基因（tfx）的快生型大豆根瘤菌H12－2（pXK）和H12－2（p3K）为供试菌株，以出发菌H12－2和8株对三叶草素敏感的快生型大豆根瘤菌混合群体为对照菌，分别在盆栽条件下进行竞争结瘤试验。结果表明，产素菌H12－2（pXK）在两个大豆品种上的占瘤率均高于不产素的重组菌H12－2（p3K）。以H12－2为对照菌时，H12－2（pXK）占瘤率比H12－2（p3K）高出22％～26％；以8株快生型大豆根瘤菌混合群体为对照菌时，可提高30％～34％。Comparative studies on the nodulation cometitiveness of two Sinorhizobium fredii strains containing tfx genes were carried out in sand pot experiment.It was found that trifolitoxin - production strain H12 - 2 (pXK) showed nigher nodulation competitiveness than trifolitoxin - immune strain H12 - 2 (p3K). H12 - 2 (pXK)showed 22 % ~26 % increase in noduLle occupancy comparing with H12 - 2 (p3K) by using H12 - 2 as reference strain and 30% ~34% increase by using 8 mixed strains of S. fredii which were senstive to trifolitoxin.There were no significant difference among two transconjugants and H12 - 2 according to the results of symbiotic nitrogen fixation efficency experiment.

关 键 词：快生型 大豆根瘤菌 竞争结瘤 占瘤率 三叶草素 

分 类 号：S154.381[农业科学—土壤学]