Zmu─1:DHP豚鼠生化基因位点多态性研究  

Study on Polymorphism of Biochemical Markers in Zmu-1:DHP Guinea Pig

作　　者：刘迪文[1] 郭汉身[1] 傅军[1] 张水利 胡根林[1] 

机构地区：[1]浙江医科大学实验动物中心,杭州310031

出　　处：《上海实验动物科学》1998年第3期129-132,共4页Shanghai Laboratory Animal Science

基　　金：浙江省卫生厅医药卫生科学研究基金

摘　　要：为了研究Zmu－1：DHP育成品系豚鼠的遗传特征，以该品系亲本DHP（暂定名Zmu－2：DHP）豚鼠作对照，采用生化电泳法测定了14个同工酶的遗传标记。结果显示了二个品系的部分生化遗传概貌。Zmu－1：DHP品系10个基因位点无多态性，而Zmu－2：DHP品系有9个。二个品系的G－3－PD，G－6－PD、ES－6和ES－8位点呈多态性，DPH品系还有GOT-1位点呈多态性。上述5个多态性位点均有a、b二个等位基因，除此之外，Zmu—2：DHP品系在Es－8位点还存在c等位基因。从结果还看出，G－6－PD位点的等位基因类型与豚鼠的性别有关，多数雄性豚鼠呈b基因，多数雌性豚鼠则呈a基因。此外，还计算了5个多态性位点各种等位基因的频率。To study the genetic characteristics of Zmu-1: DHP guinea pig, 14 biochemical markers were analysed by electrophoretic method,and compared with those of the parent DHP strain. The results reveals that no polymorphism is showed on 10 loci in Zmu-1: DHP strain and 9 loci in DHP strain. Both strains manifest polymorphism on G-3-PD,G -6-PD, Es-6 and Es- 8 loci, and GOT- 1 locus in DHP strain is also polymorphic. There are a and b alleles on above 5 polymorphic loci and beside there is c allele on Es- 8 locus in DHP strain. It also shows that the genotype on G - 6 - PD locus is sex related, most male guinea pigs possess b allele on G - 6 - PD locus while most females possess a allele on the same locus.

关 键 词：Zmu-1 DHP豚鼠 生化标记 同工酶 多态性 

分 类 号：Q959.837[生物学—动物学]