电刺激诱导兔卵孤雌活化的研究  

Study of Parthenogenetic Activation of Rabbit Eggs by Electric Stimulation

作　　者：李劲松[1] 刘辉[1] 王敏康[1] 廉莉[1] 李厚达 陈大元[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京100080

出　　处：《上海实验动物科学》1998年第3期133-136,共4页Shanghai Laboratory Animal Science

基　　金：国家八六三计划重点资助项目

摘　　要：为了给兔核移植研究提供最佳的兔卵电激活条件，研究了影响电刺激诱导兔卵孤雌活化的3个因素：细胞松弛素（CB）对卵的预处理，电极距离及脉冲强度。结果表明，兔卵经CB（7．5mg／L）预处理1h后，激活率（79．4％）显著高于对照组（48．1％，P＜0．05）；在距离为0．50mm的两电极间进行脉冲刺激，对兔卵的激活率（79．4％）显著高于电极距离为0．25mm（63．1％，P＜0．05）及1．00mm组（35．0％，P＜0．05）；另外，与1．8、2．4、3．6kV／cm（50μs）的脉冲强度相比，用3．0kV／cm、50μs的脉冲刺激后，可获得相对高的卵激活率（90．9％）和发育率（47．5％）。Three factors,cytochalasin B (CB) pre - treatment,width of two electrodes and field strength of electric pulse(direct current)related to the parthenogenetic activation of rabbit eggs by electric stimulation were studied for nuclear transplantation. The results showed that the treatment of oocytes with 7. 5 mg/L CB for 1 h followed by activation with elec- trical stimulation significantly improved the oocyte activation rate as compared with the oocytes without CB pre- treatment (79. 4% vs 48. 1%,P<0. 05). The activation rate of oocytes which were placed between the two electrodes with 0. 50mm apart was signifi- cantly improved as compared with the two electrodes with 0. 25 or 1. 00mm apart (79) 4% vs 63. 1 % or 35. 0%,P<0. 05 ). Furthermore,a high activation rate(90. 9% ) and develop- ment rate (47. 5%) were achieved when oocytes were activated electrically with a field strength of 3. 0 kV/cm (50μs) versus 1. 8,2. 4, 3. 6 kV/cm.

关 键 词：兔卵 孤雌活化 电刺激 核移植 

分 类 号：Q959.836[生物学—动物学]