柱层析法纯化人用地鼠肾细胞狂犬病疫苗  

Purification of Human Rabies Vaccine of Primary Hamster Kidney Cell

作　　者：杨立清[1] 张文璇[1] 张月兰 王福录 闻仲权[1] 

机构地区：[1]卫生部北京生物制品研究所

出　　处：《微生物学免疫学进展》1998年第3期18-20,共3页Progress In Microbiology and Immunology

摘　　要：用Ｓｅｐｈａｒｏｓｅ－６Ｂ凝胶层析法纯化原代地鼠肾细胞狂犬病苗，可去除绝大部分杂蛋白，总蛋白含量减少９９．４％以上，纯化后抗原比活性提高２１０倍，单位疫苗剂量中牛血清含量降低１８．７倍以上。方法简单，价廉。After twenty folods concentrated,Human rabies vaccine prepared from primary hamster kidney cell was purified on a sepharose6B agrose chromatography columm,The final product was purified to be 212-folds,contain of 14.5μg/IU of protein and no more than 1.94ng/IU of fetal calf serum.The recovery was aboout 100%.This method can be used in large-scale production.

关 键 词：柱层析 地鼠肾细胞 狂犬病疫苗 

分 类 号：R512.99[医药卫生—内科学]