杀白细胞毒素基因阳性耐甲氧西林金黄色葡萄球菌的红霉素耐药基因的检测  

Detection of erythromycin resistance gene in Panton-Valentine Leukocidin-positive Methicillin-resistant Staphylococcus aureus

作　　者：张征[1] 孙静娜[1] 王政民[1] 李亚威[1] 赵建宏[2] 

机构地区：[1]河北医科大学第一医院,河北石家庄050031 [2]河北医科大学第二医院,河北石家庄050000

出　　处：《现代中西医结合杂志》2010年第1期24-26,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省科技厅资助项目(052761136);河北省卫生厅重点课题计划项目(06197)

摘　　要：目的了解杀白细胞毒素(PVL)基因阳性耐甲氧西林金黄色葡萄球菌(MR-SA)的红霉素耐药基因(ermA/B/C)检出情况。方法收集2007年1月—2008年6月分离自河北医科大学第一医院临床患者的MRSA 45株,采用聚合酶链反应对45株MRSA的ermA/B/C和PVL基因进行检测,分析PVL基因阳性MRSA的ermA/B/C检出率。结果45株MRSA中有33株检出ermA/B/C,检出率为73%(33/45);有12株检出PVL基因,检出率为27%(12/45);12株PVL基因阳性MRSA中有9株检出ermA/B/C,检出率为75%(9/12);33株PVL基因阴性MRSA中仅24株检出ermA/B/C,检出率为73%(24/33)。结论PVL基因阳性与PVL基因阴性MRSA的ermA/B/C检出率基本一致,MRSA的重要致病基因——PVL的产生可能与红霉素耐药基因ermA/B/C无关。Objective It is to learn the erythromycin resistance gene（ermA/B/C） in the Panton-Valentine Leukocidin（PVL） gene-positive Methicillin-resistant Staphylococcus aureus（MRSA）.Methods 45 strains of MRSA were clinically isolated from the patients in the First Hospital of Hebei Medical University from January 2007 to June 2008.ErmA/B/C and PVL gene were detected by polymerase chain reaction（PCR） methods,and the positive rate of ermA/B/C in MRSA with positive PVL gene.Results Among 45 strains of MRSA,the positive rate of ermA/B/C was 73%（33/45）, the positive rate of PVL gene was 27%（12/45）.Among 12 strains of PVL gene-positive MRSA,the positive rate of ermA/B/C were 75%（9/12）.Among 33 strains of PVL gene-negative MRSA,the positive rate of ermA/B/C were 73%（24/33）.Conclusion The PVL gene-positive MRSA for ermA/B/C are basically in accordance with the PVL gene-negative MRSA.It shows that the production of the important pathogenic gene-PVL gene in MRSA probably has nothing to do with the erythromycin resistant gene ermA/B/C.

关 键 词：耐甲氧西林金黄色葡萄球菌 红霉素耐药基因 erm基因 杀白细胞毒素基因 PVL基因 

分 类 号：R446.5[医药卫生—诊断学]