槐树组织培养中超度含水态苗发生与防止的研究  

STUDIES ON THE OCCURRENCE AND PREVENTION OF HYPERIIYDRIC SHOOT IN TISSUE CULTURE OF SOPHORA JAPONICA

作　　者：王喆之[1] 胡正海[2] 

机构地区：[1]陕西师范大学生物系,西安710062 [2]西北大学植物研究所,西安710069

出　　处：《应用与环境生物学报》1998年第3期227-231,共5页Chinese Journal of Applied and Environmental Biology

基　　金：陕西省自然科学基金

摘　　要：对槐树（Sophorajaponica）花药愈伤组织在培养条件下形成超度含水态苗的条件研究表明：短期培养的愈伤组织及在含有较低ρ（BA）的培养基上多形成正常苗，而经长期培养的愈伤组织及在含有较高ρ（BA）的培养基上产生了大量的超度含水态苗；在MS附加ρ（IAA）0．2mg／L、ρ（BA）5．0mg／L的培养基上，愈伤组织经连续1．5a以上的继代培养后，其表面产生的芽均为超度含水态芽；将这种愈伤组织和超度含水态芽转人含不同浓度和种类植物激素及生长调节剂的培养基后发现：BA和高ρ（KT）明显促进了超度含水态苗的形成；而一定ρ（NAA）和ρ（2，4-D）则显著地促进了正常苗的形成．对培养过程的观察显示：在各种培养基上形成的正常菌中，有一部分是由超度含水态的芽和苗转化而来，即由其顶端或腋生分生组织进一步发育转化而来，而另一些则由愈伤组织上重新产生．The factors of affecting hyperhydric shoot formation from the anther calli of Sophora japonica L. were studied. The results showed that most of the regenerated shoots produced from the short-term cultured calli and from the calli cultured on the medium supplemented with lower concentration of BA were normal shoots, whereas many hyperhydric shoots were produced from the long-term cultured calli and the calli cultured on the medium with higher concentration Of BA. After one and a half years' subculture on the MS medium supplemented with ρ(IAA) 0.2mg/L and ρ(BA) 5.0mg/L, an the buds produced from the surface of the calli were hyperhydric buds .when those buds with cant were transferred to and cultured on MS medium with different concentrations,phytohormones and growth regUlators for a month, it was found that the BA and high concentration of KT markedly promoted the formation of hyperhydric shoots; but some concentrations of NAA or 2,4-D could promote the formation of normal shoots. The observation showed that some of the normal shoots were developed from the apical and axillary meristems of hyperhydric buds, and the others produced from the calli.

关 键 词：槐树 超度含水态苗 植物激素 生长调节剂 

分 类 号：S792.260.5[农业科学—林木遗传育种] Q944.6[农业科学—林学]