杉木CAD基因生物信息学分析  

Bioinformatics analysis of CAD gene from Cunninghamia lanceolata(Lamb.)Hook

作　　者：佘朝文[1,2,3] 许栋[1] 张青桦[1] 蒋向辉[1,2,3] 

机构地区：[1]怀化学院生命科学系 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室 [3]怀化学院湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《怀化学院学报》2009年第11期17-21,共5页Journal of Huaihua University

基　　金：湖南省教育厅重点资助项目(06A054);湖南省科研计划重点资助项目(2009FJ2008);民族药用植物资源研究与利用湖南省重点实验室项目(SYSXM200909)

摘　　要：杉木CAD片段为信息探针,利用序列拼接方法获得了738bp的cDNA序列.利用MEGA4.0软件对不同进化层次的代表植物相应CAD基因进行比对,构建系统进化树.利用生物信息学方法分析了杉木CAD基因的结构与功能,其中包括核苷酸序列的组成、ORF识别、CpG岛、限制性酶切位点分析、卷曲螺旋、亲水性、糖基位点及二级结构.结果表明:杉木与铁杉的CAD基因具有99.00%的同源性;该序列在88～737bp片段上有一个开放性阅读框,该序列编码的是一个碱性蛋白,亲水性较强,疏水性较弱,信号肽序列为第1位～第11位,二级结构以α-螺旋(27.50%)与不规则盘绕(41.50%)为主,延伸链(22.50%)也是该蛋白的重要结构元件,β转角区域仅为8.50%.Using CAD gene cDNA fragment as probe, 738 bp cDNA sequence was obtained by splicing. A phylogenetic tree of the homologous gene was constructed using MEGA 4. 0. Various bioinformatic methods were used to analyze and predict the feature and possible functions of the CAD gene cDNA sequence, including composition of nucleic acid, ORF finder, CpG Island finder, theoretical pI, alienation/proximity water area, secondary structure, et al.. the results showed the homology based CAD gene was 99.00% between Cunninghamia lanceolata （Lamb.） Hook and Nothotsuga longibracteata ; the sequence contained one ORF during 88 - 737 bp, the protein with more hydrophilic and less hydrophobicity, signal peptide locate in section 1 to section 11, in the structure α- helix and random coil were 27.50% and 41.50% respectively, also extended strand were important construction component of the protein, the percentage of β- angle region was only 8.50%.

关 键 词：杉木 CAD基因 生物信息学 

分 类 号：Q785[生物学—分子生物学]