新加良附方对移植性人胃癌细胞Survivin与Caspase-3蛋白表达影响  

The Effects of Xinjialiangfu Formula on Survivin and Caspase-3 Expression of Transplanted Human Gastric Cancer Cell Line (BGC-823)

作　　者：田劭丹[1] 董青[1] 侯丽[1] 陈信义[1] 

机构地区：[1]北京中医药大学东直门医院肿瘤血液科,北京100700

出　　处：《现代生物医学进展》2009年第21期4021-4023,4065,F0002,共5页Progress in Modern Biomedicine

基　　金：教育部高等学校博士点基金(新教师基金)资助(编号:200800261007)

摘　　要：目的:观察新加良附方对移植性人胃癌细胞(BGC-823)Survivin与Caspase-3蛋白表达的影响。方法:建立移植性人胃癌动物模型,并将动物模型随机分为模型对照、5-Fu组及新加良附方高、中、低剂量5组。新加良附大、中、小剂量组给药量分别为10g/kg、5g/kg和2.5g/kg;5-Fu组给药剂量为17mg/kg;模型组给予等量无菌生理盐水。连续给药、给水15天处死模型小鼠分离肿瘤,瘤块称重,石蜡包埋,并将肿瘤组织切片,免疫组化法检测Survivin与Caspase-3蛋白表达。结果:新加良附方高、中剂量组能上调肿瘤组织Caspase蛋白表达,与模型对照组比较,有统计学意义(P<0.05);新加良附方高、低剂量组均能下调Survivin蛋白表达,与模型对照组比较,有统计学意义(P<0.05)。结论:新加良附方能通过上调肿瘤组织Caspase蛋白表达,下调Survivin蛋白表达,从而诱导胃癌细胞发生凋亡。预示新加良附方在抗肿瘤方面具有进一步深入研究价值。Objective：To observe the effects of Xinjialiangfu Formula（XJLFF） on Survivin and Caspase-3 expression of transplanted human gastric cancer cell line（BGC-823） . Methods：Animal models with human gastric cancer xenografts were established and randomly divided into 5 groups;namely model control group,5-Fu group,XJLFF high-dose group,XJLFF medium-dose group and XJLFF low-dose group. XJLFF groups of high,medium and low dosage were administered XJLFF at the amount of 10g/kg,5g/kg and 2.5g/kg respectively;while 5-Fu group was administered 5-Fu at a dose of 17mg/kg,and the model control group was given equivalent sterile normal saline. All mice were sacrificed after 15 days of continuous drug and water delivery. The tumor blocks were separated,weighed,embedded in paraffin,dissected into slices,and Survivin and Caspase-3 expression was detected by immunohistochemical method. Results：Caspase-3 expression in tumor tissue of XJLFF high-dose and medium-dose group was up-regulated,and there was statistically significant difference compared with model control group（P〈0.05） . Survivin expression in tumor tissue of XJLFF high-dose and low-dose group was down-regulated,and it was also significantly different from that in model control group（P〈0.05） . Conclusion：The XJLFF can up-regulate Caspase-3 expression and down-regulate Survivin expression in tumor tissue by inducing apoptosis in gastric cancer cells. This finding indicates that an in-depth research on the anti-tumor effect of XJLFF is in need and worthwhile.

关 键 词：新加良附方 移植性人胃癌细胞 凋亡机制 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]