树突状细胞和抗原负载树突状细胞在肿瘤模型鼠体内的分布和形态学观察  

Distribution and Morphological Observation of DC and Antigen-loaded DC in Tumor Mouse Model in Vivo

作　　者：鲁占军[1] 姜哲 宋承鑫[1] 贾立敏[1] 贺业春[1] 谢遵江[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150081 [2]黑龙江护理高等专科学校,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2009年第21期4033-4036,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科研项目(11511186)

摘　　要：目的:研究体外培养的小鼠树突状细胞(dentritic cells,DCs)和抗原负载树突状细胞在肿瘤模型鼠体内的分布和形态,为肿瘤的生物学治疗提供形态学基础。方法:分离和培养DC,制备B16黑色素瘤细胞抗原,进行共培养,即为抗原负载的DC,Brdu标记DC和抗原负载的DC,建立B16黑色素瘤小鼠模型,于瘤周围皮下注射Brdu标记的DC和抗原负载的DC。应用光镜、免疫组化方法和透射电镜观察DC和抗原负载DC在肿瘤模型鼠体内的分布和形态。结果:免疫组化染色显示Brdu标记的抗原负载DC与DC比较,体积较大。实验组Brdu标记的DC和抗原负载DC分布的数密度和面密度,分别与对照组比较,有显著差异(P<0.01)。电镜下抗原负载DC细胞与DC比较体积较大,核有切迹,细胞表面的突起较粗大弯曲,形态较成熟。结论:抗原负载DC比DC更易集聚于肿瘤组织周围,推测抗原负载DC比DC可能诱导抗肿瘤效应更强。Objective：To investigate the distribution and morphology of the cultured dentritic cell（DC） and antigen-loaded DC in tumor mouse model in Vivo,provide morphological basis for the tumor biological therapy. Methods：DCs were separated and cultured. antigen was extracted from B16 melanoma cells by method of freeze thawing. They were co-cultured. The co-cultured cells were antigen-loaded DC. DC and antigen-loaded DC were marked by Brdu. The mouse model of B16 melanoma was established. Brdu-marked DC and antigen-loaded DC were injected into the tumor-surrounding tissues. The distribution and morphology of DC and antigen-loaded DC in tumor mouse model in Vivo was observed by optical microscopy,electron microscopy and immunohisochemistry. Results：The antigen-loaded DC cells were bigger than the DC cells. The number（area） density of Brdu-marked DC and antigen-loaded DC was significantly higher in the model group than that in the controls respectively（P〈0.01） . Under electron microscopy,the antigen-loaded DC cells were bigger than the DC cells and notch which always appears on cellular nucleus was observed. The protrusions on the surface of antigen-loaded DC cells were more grossus and curved than that of DC,and their morphology was more mature. Conclusion：The antigen-loaded DC cells were more accumulated to the tumor-surrounding tissues compare with DC. These suggested that the role of against tumor induced antigen-loaded DC was stronger than that of DC.

关 键 词：小鼠 树突状细胞 抗原负载树突状细胞 肿瘤 形态学 

分 类 号：R392.12[医药卫生—免疫学]