乙肝HBV DNA荧光定量检测与血清标志物结果相关性  

The relationship of quantitative detection of hepatitis B DNA fluorescence and serum marker rest results

作　　者：钱东林 

机构地区：[1]江苏省吴江市第一人民医院,215200

出　　处：《临床合理用药杂志》2009年第24期13-14,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的研究乙肝血清学标志物(HBV-M)与HBV-DNA水平的关系,分析HBV-DNA与乙肝5项的相关性。方法随机抽取的门诊和住院患者血清502份,应用荧光定量PCR检测HBV-DNA和免疫化学发光法检测乙肝血清学标志物并对结果进行分析。结果259份HBV-DNA阳性。其中123份1.3.5阳性(大三阳)血清中HBV-DNA的阳性率为92.48%;113份1.4.5阳性(小三阳)血清中HBV-DNA的阳性率为48.09%;12份1.5阳性血清中HBV-DNA的阳性率为40.00%;11份1.3.4.5阳性血清中HBV-DNA的阳性率为78.57%,大三阳的阳性率与PCR-DNA的阳性率差异有统计学意义(P<0.01)。结论HBV-M与HBV-DNA两者之间互有联系但又有不同。对乙型肝炎患者进行多项指标的联合检测,对于临床判断其传染性及治疗效果非常必要。Objective To analyze the relationship between the level of HBV-DNA and the HBV-M. Methods Serum samples from 502 service workers were detected by immuntv of shine and determined by fluorescent quantitative PCR. Results The positive rate of HBV-DNA in the group of HBsAg（ ＋ ）/HBeAg（ ＋ ）/HBcAb（ ＋ ）was 92.48% , the positive rate in the group of HBsAg（ ＋ ）/HBeAb（ ＋ ）/HBcAb（ ＋ ）was 48.09% ,the positive rate in the group of HBsAg（ ＋ ）/HBcAb（ ＋ ）was 40.00% ,78.57% in the group of HBsAg（ ＋ ）/HBeAg（ ＋ ）/HBeAb（ ＋ ）/HBcAb（ ＋ ）. There was significant differences between the different hepatitis B sera positive modes and the result of HBV-DNA（P 〈 0.01 ）. The HBV-DNA level was showed an obvious correlation with the HBeAg content in sera. Conclusion However, the relationship between HBV-M and HBV- DNA are manifest,as well as their difference. It is necessary to make clinical judgement with infectiousness and therapeutic efficacy of type B hepatitis sufferers by muhi-indicatrix from combined detection.

关 键 词：乙肝病毒血清标志物 HBV—DNA 免疫化学发光法 荧光定量PCR 相关性 

分 类 号：R446.6[医药卫生—诊断学]