PolyI∶C不同途径诱导大菱鲆Mx蛋白基因的转录  

Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic-polycytidylic acid given in different ways

作　　者：王印庚[1] 毛明光[1,2] 刘宗柱[3] 张家松[1] 陈贵平[1] 廖梅杰[1] 曲江波 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]厦门大学海洋与环境学院,361005 [3]青岛农业大学生命科学学院,266109 [4]烟台开发区天源水产有限公司,264000

出　　处：《渔业科学进展》2009年第6期1-6,共6页Progress in Fishery Sciences

基　　金：国家863计划(2003AA622070);国家十一五科技支撑计划(2006BAD09A11);行业专项(nyhyzx07-046)共同资助

摘　　要：以PolyI∶C(又称聚肌胞)为诱导剂,分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆Scophthalmus maximus体内抗病毒蛋白Mx基因的转录,利用半定量RT-PCR方法检测干扰素下游基因-Mx基因的转录水平来确定该诱导剂的诱导效果。结果显示,以上3种途径都能高效诱导Mx蛋白mRNA的转录,均在48h之后达到高峰,其中以浸泡的方式更容易诱导Mx基因转录,且在120h时仍保持较高水平。Mx基因转录的时相变化证明了国产PolyI∶C可以通过多种途径诱导抗病毒蛋白Mx的表达,为实际应用中确定用药途径提供了理论依据。另外,实验初步建立了半定量RT-PCR方法,为检测鱼体内干扰素的表达提供了技术方法。Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI ： C with intraperitoneal injection, immersion and oral administration. Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI ： C＇s induction. The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI ： C application. The immersion method seemed to be the most effective way to induce Mx mRNA, as Mx mRNA could last more than 120h at a high level. This experiment proved that the homemade PolyI ： C could effectively induce Mx mRNA transcription in different applications, which provided a reference to use PolyI ： C in aquaculture practice.

关 键 词：PolyI∶C 大菱鲆 MX蛋白 干扰素诱导剂 基因转录 

分 类 号：S942.5[农业科学—水产养殖]