半滑舌鳎精子冷冻保存  

Cryopreservation of tongue sole Cynoglossus semilaevis sperm

作　　者：田永胜[1] 陈松林[1] 季相山[1] 孙礼娟[1] 翟介明[1] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《渔业科学进展》2009年第6期97-102,共6页Progress in Fishery Sciences

基　　金：国家自然科学基金项目(30570259);"国家"863项目(2006AA10A403);农业科技成果转化资金项目(2007GB2C600174);农业部海洋渔业资源可持续利用重点开放实验室开放课题(实开2005-03)共同资助

摘　　要：半滑舌鳎精子冷冻保存对于人工繁殖育苗、杂交育种、雌核发育及其性别控制研究具有重要的意义,为此,本文对半滑舌鳎精子冷冻保存方法进行了研究。分别利用2.8mol/L的二甲基亚砜(DMSO)、甘油(Gly)和1,2-丙二醇(PG)冷冻保存该鱼精子。结果显示,DMSO冷冻保存精子的活力较高。利用MPRS+2.8mol/L DMSO以1∶0.5、1∶1、1∶1.5和1∶2的比例稀释并冷冻精子,1∶1比例在冻前能够抑制精子的运动,冻后活力可达82.50±3.54%,显著高于其他稀释比例(P<0.05)。分别利用冷冻保存液A(MPRS+2.8mol/L DMSO)和B(TS-2+2.8mol/L DMSO)稀释平衡精子,精子在A中的冻前快速运动时间、寿命分别为37.75±6.45s和145.00±78.98s,与鲜精无显著差异(P>0.05)。利用以上两种冷冻稀释液冷冻保存精子,精子在A液中的冻后活力和寿命分别可达53.50±6.69%和98.00±13.51s,冷冻效果优于B液(P<0.05)。冷冻后精子的受精率和孵化率分别为55.00±5.00%和35.00±13.23%,受精率与鲜精无显著性差异(P>0.05),因此认为MPRS+2.8mol/LDMSO可用于半滑舌鳎精子的冷冻保存。Cryopreservation of tongue sole Cynoglossus semilaevis sperm offers benefits for artificial propagation, hybridization, gynogenesis and sex control. In order to develop a protocol to freeze tongue sole sperm, the effects of cryoprotectants, extenders and dilution ratio on motility, fertilization rate and hatching rate of frozen-thawed sperm were evaluated. Dimethylsul- foxide（DMSO） provided higher protection than glycerol and 1,2-propylene glycol during freezing and thawing. Motion time and life of sperm in A（MPRS ＋2.8 mol/L DMSO）and B（TS-2＋2.8 mol/L DMSO）before freezing were 37.75±6.45 sec and 145.00±78.98 sec,which was not different from that of fresh sperm（P〉0.05）. The motility and life of frozen-thawed sperm in A were 53. 50±6. 69% and 98.00±13.51 sec,which was significantly higher than that of frozenthawed sperm in B. When sperm were diluted with A at a ratio of 1 ： 0.5,1 ： 1,1 ： 1.5 and 1 ：2,the highest motility（82.5±3.54%, 1 ：1 dilution ratio）of frozen thawed sperm was obtained and which was significantly higher than that of other dilution ratio treatment. According to theresults above, tongue sole sperm were cryopreserved successfully with MPRS ＋2. 8 mol/L DMSO at a dilution ratio of 1 ： 1. As a result,the fertilization rate（55.0±5.0%）and hatchingrate（35 ± 13.23 %） of frozen-thawed sperm were obtained, which was not significantly different from that of fresh sperm（P〉0. 05）.

关 键 词：半滑舌鳎 精子 冷冻保存 

分 类 号：Q65[生物学—生物物理学] Q959.4