正交试验法优选鬼臼毒素新型脂质体的制备工艺  

Study on the optimum preparation condition of the new type podophyllotoxin liposome with orthogonal design

作　　者：程冀[1,2] 季宇彬[1,2] 王向涛[3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《黑龙江大学自然科学学报》2009年第6期795-798,808,共5页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(30772659)

摘　　要：确立鬼臼毒素新型脂质体的最佳制备工艺。采用正交设计方法对最佳制备工艺进行实验,以氢化磷脂与胆固醇比例、氢化磷脂与PAMAM dendrimer G3.5脂质小球的比例、氢化磷脂与鬼臼毒素的比例、旋转蒸发温度为4个因素,包封率为考察指标,筛选出鬼臼毒素新型脂质体最佳制备工艺。优选出鬼臼毒素新型脂质体的制备工艺为氢化磷脂∶胆固醇=20∶3.26,氢化磷脂∶PAMA Mdendrimer G3.5脂质小球=20∶0.5,氢化磷脂∶鬼臼毒素=20∶1.5,旋转蒸发温度为45℃。所选工艺制备的成品符合脂质体的质量标准。To select the optimum condition of the new type podophyllotoxin of the new type podophyllotoxin liposome preparation was performed based The entrapment efficiency of the new type podophyllotoxin liposome were liposome preparation. The optimization on the orthogonal experimental design. taken as the index and the influence of factors such as the proportion of hydrogenated phospholipid and Cholesterol, the proportion of hydrogenated phospholipid and PAMAM dendrimer G3.5 lipid globule, the proportion of hydrogenated phospholipid and podophyllotoxin and the temperature of rotary evaporation. The best formulation of the new type podophyllotoxin liposome were the proportion of hydrogenated phospholipid and Cholesterol was 20 ： 3.26, the proportion of hydrogenated phospholipid and PAMAM dendrimer G3.5 lipid globule was 20 ： 0. 5, the proportion of hydrogenated phospholipid and podophyllotoxin was 20 ： 1.5 and the temperature of rotary evaporation was 45℃. The new type podophyllotoxin liposome designed according to orthogonal experiment was coincidenced with the requirements in aspect of the liposome.

关 键 词：鬼臼毒素 新型脂质体 正交设计实验 

分 类 号：R944[医药卫生—药剂学]