微分化型急性髓系白血病5例临床分析  

Clinical Analysis of 5 Cases With Minimally Differentiated Acute Myeloblastic Leukemia

作　　者：吴学琼[1] 田野[1] 王瑾[1] 李春蕊[1] 孙汉英[1] 周剑锋[1] 刘文励[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2009年第6期310-312,共3页Journal of Critical Care In Internal Medicine

摘　　要：目的:探讨流式细胞免疫分型在微分化型急性髓系白血病(AML-M0)诊断中的作用。方法:分析5例AML-M0患者的临床资料,观察其骨髓细胞形态学和细胞化学染色的特征以及与流式细胞仪骨髓细胞免疫分型的符合程度。结果:5例患者骨髓细胞学均报告原始及幼稚淋巴细胞增多,POX染色阴性,PAS染色部分阳性,流式细胞免疫分型髓系标志CD13、CD33、CD117至少1项阳性,而淋巴系统及巨核细胞系统抗原阴性。结论:骨髓细胞形态学、细胞化学染色结合骨髓细胞免疫表型和细胞遗传学检查是诊断AML-M0的主要依据,其中原始细胞的免疫分型对诊断AML-M0是非常必要的。Objective. To investigate the role of immunophenotyping by tlow eytometry（FCM） xn magnosis of minimally differentiated acute myeloblastic leukemia. Methods： Clinical data of 5 cases with minimally differentiated acute myeloblastie leukemia were analyzed. The bone marrow morpbocytology, characteristics of cytochemical staining and bone marrow immunophenotype by FCM were observed. Results： All 5 cases showed that primitive and immature lymphocytes in bone marrow were increased with POX staining negative and PAS staining partly positive, at least one of myeloid antigens such as CD13,CD33 and CD117 was expressed, while lymphocyte and megakaryocyte antigens were negative. Conclusions： It＇s important in diagnosis of minimally differentiated acute myeloblastie leukemia by bone marrow morphoeytology, cytochemical staining combined with immunophenotyping and eytogenics, while immunophenotyping was the most necessary.

关 键 词：髓系白血病 微分化型 流式细胞术 免疫表型 

分 类 号：R551[医药卫生—血液循环系统疾病]