甘蓝型油菜显性核不育橙黄花纯合两型系的选育  

Developing a homozygous two-type line of dominant genic male sterility with orange flower in Brassica napus L.

作　　者：周熙荣[1] 庄静[1] 孙超才[1] 王伟荣[1] 李延莉[1] 顾龙弟[1] 钱小芳[1] 

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《中国油料作物学报》2009年第4期421-425,共5页Chinese Journal of Oil Crop Sciences

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2008)第7-3号]

摘　　要：以甘蓝型油菜显性核不育杂合两型系的不育株沪油15A为母本,与恢复系HF03杂交,F1群体全部表现为可育。在该杂交组合的6个F3群体中有5个群体发生育性分离。其中区号为04-4307的F3群体发生育性和花瓣色泽的分离,表现为70株鲜黄花可育、22株鲜黄花不育、30株橙黄花可育、9株橙黄花不育。根据油菜显性核不育和橙黄花的遗传规律,04-4307出现3∶1育性分离,说明该群体由基因型为M sM sR frf的单株自交获得。从04-4307群体中选橙黄花可育株自交,并与橙黄花不育株作兄妹交,后代全部表现为橙黄花(y1y1y2y2)。其中区号为05-4356的兄妹交组合出现1∶1育性分离;其父本的可育株自交出现3∶1育性分离。由此推断,05-4356的兄妹交组合就是显性核不育橙黄花纯合两型系,其不育株基因型为M sM srfrfy1y1y2y2,可育株基因型为M sM sR fr-fy1y1y2y2。A cross between heterozygous two-type line Huyou15A of dominant genic male sterility and restorer line HF03 led to all fertile F1 plants. Five among 6 F3 generation lines showed fertile segregation. A registered 04- 4307 group showed a fertility segregation ratio at 3： 1, including 70 fertile with yellow flowers, 30 fertile with orange flowers,22 sterile with yellow flowers and 9 sterile with orange flowers. The genotype of its F2 plant should be MsMsRfrf. The fertile plants with orange flowers were self-pollinated and crossed with sister sterile plants with orange flowers. Both led to all orange flower offspring, while the sib-mating generation 05-4356 showed a fertility segregation ratio at 1： 1, and the self generation of corresponding fertile plant segregated at 3： 1. These results indicated the 05-4356 group to be a homozygous two-type line with orange flower developed by crossing sterile genotype MsMsrfrfy1y1y2y2 and fertile genotype MsMsRfrfy1y1y2y2.

关 键 词：甘蓝型油菜 显性核不育 纯合两型系 临保系 恢复系 橙黄花 

分 类 号：S565.403[农业科学—作物学]