重庆地区部分牛和猪外周血博尔纳病病毒自然感染的调查  

Investigation of natural Borna disease virus infection in peripheral blood mononuclear cells of some cattle and pigs in Chongqing

作　　者：张英英[1] 徐鸣明[1] 展群岭[1] 余建萍[1] 翟红[1] 刘妍汐[1] 陈晓[1] 彭丹[1] 朱丹[1] 谢鹏[1,2] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]重庆医科大学神经科学研究中心,重庆400016

出　　处：《中国兽医科学》2009年第12期1045-1050,共6页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2006AA02Z196)

摘　　要：采用荧光定量巢式实时逆转录聚合酶链反应检测了重庆地区牛和猪各50例外周血单核细胞中博尔纳病病毒(Borna disease virus,BDV)p24基因片段,并对其进行BDV p40基因片段的检测以确保结果的可靠性。检出的阳性序列与GenBank中5个国家4个动物种属25例BDV p24基因序列进行比对,分析其核苷酸和氨基酸序列的同源性,重建基因系统发生树。结果显示,3例牛和2例猪血标本BDV p24检测呈阳性,阳性率分别为6.00%和4.00%;5例标本BDV p40片段检测均为阳性,其余标本均为阴性;BDVp24扩增产物序列与BDV标准病毒株He/80的同源性为100%,与H1766和Strain V相比,变异程度小,所编码的氨基酸序列亦无变化;从发生树可以看出,5例BDV p24片段序列汇聚德国-瑞士-奥地利-日本-重庆混合支系。提示,重庆地区牛和猪中可能存在BDV的自然感染,其流行株可能源于外来疫病病原的传入。Borna disease virus（BDV） p24 gene fragment was detected by fluorescence quantitative nested real-time RT-PCR from peripheral blood mononuclear cells（PBMCs） of fifty healthy cattle and fifty healthy pigs in Chongqing. Positive samples were repeatedly confirmed by detecting BDV p40 gene fragment. By alignment with twenty-five BDV p24 sequences of four animals in five countries in GenBank, the sequence homologous of the nucleotide and the amino acid of BDV p24 positive samples was analyzed and gene phylogenetic tree was reconstructed. The positive rates of BDV infection in PBMCs of the 50 cattle and the 50 pigs were 6.00% and 4.00%, respectively. The results of BDV p40 detection confirmed all of the samples with BDV p24 positive. The homology of the nucleotide and the amino acid between p24 amplified from samples and that of strain He/80 was 100%,and the sequence homology was more than 93% identical to those of H1766 and strain V. Five nucleotide sequences clustered within Germany,Switzerland, Austria ,Japan, Chongqing mixed branch. The results suggested that natural infection of BDV might occurred in cattle and pigs in Chongqing area, and the epidemic strain might due to the spread of foreign pathogen.

关 键 词：博尔纳病病毒 荧光定量巢式实时逆转录聚合酶链反应 p24片段 p40片段 种系发生 

分 类 号：S852.659.5[农业科学—基础兽医学]