理气开郁法板块方对炎症介质的作用特点研究  

Action characteristics of fixed Chinese herbal prescriptions of Regulating Qi to Alleviate Depression(理气开郁方) on inflammatory mediators

作　　者：孔维涵[1] 朱爱江[1] 方步武[1] 吴咸中[2] 田在善[2] 

机构地区：[1]天津医科大学,天津300170 [2]天津市中西医结合急腹症研究所,天津300100

出　　处：《中药药理与临床》2009年第5期1-3,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：天津市科技攻关计划重大科技项目(05YFGDSF02600):分项目(按治则归类进行新药开发与作用机理研究)。

摘　　要：目的:探讨理气开郁法板块方对炎症介质影响的相对特征性作用。方法:以过滤空气皮下注射和1%角叉菜胶囊内注射制备大鼠背部气囊炎症模型,用放免法检测炎性渗出液中IL-6和PGE2及血清皮质醇,用硝酸还原酶法测定渗出液中NO,预防性灌胃用药。结果:各板块方高、中、低剂量和氢化可的松均能显著降低大鼠气囊炎症渗出液中IL-6水平,金铃子散高剂量的作用有优于氢化可的松的趋势。金铃子散高、中、低剂量、芍甘汤高、中剂量、柴郁汤中剂量及氢化可的松均能显著降低大鼠气囊炎症渗出液中PGE2水平,金铃子散和芍甘汤高、中剂量的作用与氢化可的松相当。金铃子散、芍甘汤、柴郁汤高、中、低剂量、痛泻要方中剂量和氢化可的松均能显著降低大鼠气囊炎症渗出液中NO含量,金铃子散高、中、低剂量、芍甘汤高剂量、柴郁汤中、高剂量的作用尤著。模型对照组大鼠血清皮质醇水平明显升高,氢化可的松组与芍甘汤低、中剂量组血清皮质醇明显降低。结论:金铃子散、痛泻要方降低IL-6的作用较强;金铃子散、芍甘汤降低PGE2的作用较强;金铃子散和柴郁汤降低NO的作用较强;四方中金铃子散的抗炎作用可能最强;芍甘汤的抗炎作用可能还涉及到皮质醇。Objective：To study the relative characteristic action of Fixed Prescriptions of Regulating Qi to alleviate mental depression（理气开郁方） on the inflammatory mediators.Methods： The induced air pouchitis model of rats on the back was prepared by subcutaneous injection of filtered air and intrapouch injection with 1% carrageenin.The contents of cortisol in serum,IL-6 and PGE2 in the inflammatory exudate were assayed by radioimmunoassay;and NO by nitrate reductase method.Rats of air pouchitis model were treated by preventive oral administration.Results： Compared with control group at the same site,inflammatory exudate could be collected in the airbag.All the low-,moderate-and high-dose herbal prescriptions and hydrocortisone could significantly decrease IL-6 levels in the inflammatory exudate（P0.01）,and the action of high-dose Jinlingzi San（金铃子散） reducing IL-6 was stronger than that of hydrocortisone.Jinlingzi San（金铃子散） of low,moderate,high dose,Shaogan Tang（芍甘汤） of moderate and high dose,Chaiyu Tang（柴郁汤） of moderate dose and hydrocortisone could markedly reduce PGE2 level in the inflammatory exudate.Jinlingzi San（金铃子散） and moderate-and high-dose Shaogan Tang（芍甘汤） showed similar action of decreasing PGE2 to hydrocortisone.Jinlingzi San（金铃子散）,Shaogan Tang（芍甘汤）,low-,moderate-and high-dose Chaiyu Tang（柴郁汤）,moderate-dose Tongxie Yaofang（痛泻要方）,and hydrocortisone could significantly lowered NO level in the inflammatory exudate,especially low-,moderate-and high-dose Jinlingzi San（金铃子散）,hige-dose Shaogan Tang（芍甘汤）,moderate-and high-dose Chaiyu Tang（柴郁汤）.Hydrocortisone and low-and moderate-dose Shaogan Tang（芍甘汤） could significantly decrease cortisol level in serum,however,serum cortisol in the model control group significantly increased（P0.05）.Conclusion： Jinlingzi San（金铃子散） and Tongxie Yaofang（痛泻要方） have better effect of reduci

关 键 词：理气开郁法 金铃子散 芍甘汤 痛泻要方 柴郁汤 白细胞介素-6 前列腺素E2 一氧化氮 皮质醇 气囊炎症模型 

分 类 号：R285[医药卫生—中药学]