芍药苷对辐射致血虚证小鼠骨髓细胞白介素及其受体基因表达的作用  

Effect of paeoniflorin on interleukin and interleukin receptor gene expression in bone marrow cell of Xue deficiency(血虚) mice induced by irradiation

作　　者：郭平[1] 郭霞[2] 

机构地区：[1]山东中医药大学生化教研室,济南250355 [2]山东中医药大学附属医院,济南250011

出　　处：《中药药理与临床》2009年第5期25-27,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助课题(30271617)

摘　　要：目的:研究芍药苷对辐射致血虚证小鼠骨髓细胞IL-6、IL-18、IL-4R和IL-7R基因表达的影响,为阐明芍药苷补血作用的分子机制提供依据。方法:采用3.5 Gy60Coγ射线全身1次性照射制备小鼠血虚证模型,芍药苷按26.5 mg/kg.d的剂量连续灌胃给药7天;骨髓集落培养计数CFU-GM、CFU-E、BFU-E、CFU-m ix;RT-PCR检测骨髓IL-6、IL-18、IL-4R和IL-7R mRNA水平。结果:芍药苷显著升高血虚证小鼠骨髓CFU-GM、CFU-E、BFU-E、CFU-m ix数量,使其分别由7.50±3.11,71.00±11.46,8.50±1.29和7.25±2.22升至16.75±0.96,153.00±6.58,50.25±12.20和22.00±6.16;上调骨髓IL-6、IL-4R和IL-7R mRNA水平,使其与内参照的比值分别由0.26±0.02,0.19±0.04和0.11±0.02升至0.35±0.04,0.32±0.05和0.17±0.03,对IL-18 mRNA水平无显著影响。结论:芍药苷能促进骨髓造血祖细胞的增殖,上调骨髓细胞IL-6、IL-4R和IL-7R的基因表达。Objective：To observe the effect of paeoniflorin on IL-6,IL-18,IL-4R and IL-7R gene expression in bone marrow cell of Xue deficiency mice induced by radiation and to provide the foundation for explaining the blood enriching mechanism of paeoniflorin.Methods： The Xue deficiency mice were induced by using a single dose of 3.5 Gy 60Co γ radiation.Myeloid colony cell was cultured and the number of CFU-GM,CFU-E,BFU-E and CFU-mix were observed and counted.IL-6,IL-18,IL-4R and IL-7R mRNA in bone marrow were measured by RT-PCR.Results： Paeoniflorin can raise significantly the number of CFU-GM,CFU-E,BFU-E and CFU-mix in bone marrow of Xue deficiency mice and up-regulate IL-6,IL-4R and IL-7R gene expression of bone marrow cell,but it is not of significant effect on IL-18 mRNA level.Conclusion： Paeoniflorin can promote the proliferation of hematopoietic progenitor cells of bone marrow and up-regulate IL-6,IL-4R and IL-7R gene expression in bone marrow cells.

关 键 词：芍药苷 四物汤 血虚证 造血祖细胞 白介素 白介素受体 mRNA 

分 类 号：R285.5[医药卫生—中药学]