单糖的对氨基苯甲酸乙酯衍生物的毛细管电泳研究  

Capillary Zone Electrophoresis of Monosaccharides Derivatized with Ethyl-P-Aminobenzoate

作　　者：严峰[1] 徐桂云[1] 常理文[1] 

机构地区：[1]中国科学院化学研究所,100080

出　　处：《现代仪器使用与维修》1998年第6期12-14,37,共4页Modern Instruments

基　　金：国家自然科学重点基金(29635020)

摘　　要：10种单糖,包括氨基糖,中性糖和糖醛酸经对氨基苯甲酸乙酯衍生后,用硼砂缓冲液作为电泳介质,获得了全分离。应用β-吲哚乙酸作为内标,建立了10种单糖的定量方法,以葡萄糖为代表,考察了线性范围。在10mg/l～150mg/l的范围内具有良好的线性关系。最低检出浓度在0.1mM的数量级。该方法已成功地用于测定多糖的单糖组成。Ten monosaccharides derivatized with P-Aminobenzoate ethyl esters have been separated as their borate complexes by capillary zone electrophoresisusing 100mM borax as running buffer. The detector used is an on-column UV monitor at 214nm. The quantitative analysis of each monosacccharids was carried out using β-indolyl acetic acid as the internal standard. A good linear relationship between the ratio of peak area of the monosaccharide derivative to the internal standard and concentration of the monosaccharides is in the 10mg/1~150mg/l range. This method has been applied to determine the monosaccharide components of polysaccharides.

关 键 词：单糖 对氨基 苯甲酸乙酯 毛细管电泳 生物体 

分 类 号：Q532.03[生物学—生物化学]