雷公藤内酯醇对内毒素激活小鼠腹腔巨噬细胞分泌促炎递质NO和IL-6的影响  

Effect of Triptolide on Secretion of Inflammatory Cellular Factor NO and IL-6 in Mice Peritoneal Macrophage Activated by Lipopolysaccharide

作　　者：杨帆[1] 白祥军[1] 刘开俊[1] 杨业金[2] 曾叶[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院创伤外科,武汉430030 [2]华中科技大学同济医学院生化与分子生物学系,武汉430030

出　　处：《医药导报》2010年第1期9-12,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号：30070899)

摘　　要：目的观察雷公藤内酯醇(TP)对内毒素(LPS)激活小鼠腹腔巨噬细胞(MΦ)分泌促炎递质一氧化氮(NO)和白细胞介素-6(IL-6)的影响。方法分离纯化MΦ,用LPS激活,与不同浓度TP进行培养,以Griess试剂检测培养上清液中的NO含量;以ELISA法检测培养上清液中的IL-6的浓度。结果TP在浓度0.01～10.00μg·mL-1范围内,时间4～24h范围内,对MΦ产生NO具有显著抑制作用(P<0.01),且呈剂量和时间依赖关系;TP在浓度0.001～10.000μg·mL-1范围内,时间12h,对MΦ产生IL-6具有显著抑制作用(P<0.01),呈剂量依赖关系。结论TP可以抑制被LPS激活的MΦ活性,具有高效低毒的抗炎作用。Objective To study the effect of triptolide （TP） on NO and IL-6 of peritoneal elicited macrophage （MФ） activated by lipopolysaccharide（LPS） in mice. Methods The peritoneal elicited macrophage were separated, purified and activated by LPS in mice,then cultured in vitro with a series concentration of TP. The activity of NO and level of IL-6 in cellular supernatants were determined by Griess reagent and ELISA, respectively. Results The activity of NO in MФ was significantly inhibited （P 〈 0.01） by TP（0.01 - 10.00μg·mL^-1） during 4 -24 h in a time and dosage dependent manner;The level of IL-6 was obviously decreased（P 〈 0.01 ） by TP（0. 001 - 10. 000 μg·mL^-1 ） in 12 h in a dose-dependent way. Conclusion TP could inhibit the NO activity and IL-6 level in macrophage activated by LPS, and it has strong anti-inflammatory effects with low toxicity.

关 键 词：雷公藤内酯醇 内毒素 巨噬细胞 一氧化氮 白细胞介素-6 

分 类 号：R286[医药卫生—中药学] R392.114[医药卫生—中医学]