不同免疫程序和IL-18对乙型肝炎病毒PreS2-S基因DNA疫苗免疫效果的影响  

Effect of Different Immune Progress on Immune Efficacy of DNA Vaccine Expressing HBV PreS2-S Gene

作　　者：栾翔凌[1] 郭雷[2] 刘素君[3] 胡燕[4] 侯俊[4] 沈宏辉[4] 辛绍杰[4] 貌盼勇[4] 

机构地区：[1]中国人民解放军第261医院,北京100094 [2]吉林大学生命科学院 [3]北京朝阳医院西院区 [4]中国人民解放军第302医院

出　　处：《实用预防医学》2010年第1期1-3,共3页Practical Preventive Medicine

基　　金：国家自然基金项目(30840074)

摘　　要：目的探讨不同免疫程序对表达乙型肝炎病毒(HBV)PreS2-S基因的DNA疫苗免疫效果的影响。方法分别采用重组质粒DNA疫苗单独免疫、重组MVA(r MVA)载体疫苗单独免疫、DNAprime/r MVAboost、DNAprime/r MVAboost并以表达鼠IL-18(mIL-18)的真核表达质粒为佐剂的程序免疫C57BL/6小鼠,通过ELISA检测抗-HB-sAb及ELISPOT检测IFN-γ评价体液免疫和细胞免疫的水平。结果表达PreS2-S抗原的各组疫苗在抗体产生的时间和滴度方面差异均无统计学意义,用ELISPOT检测IFN-γ的产生可知,所有免疫组均可引起针对特异性抗原的细胞免疫,r MVA单独免疫组强于DNA疫苗单独免疫组,弱于DNAprime/r MVAboost和DNAprime/r MVAboost+mIL-18组,DNAprime/r MVAboost和DNAprime/r MVAboost+mIL-18之间差异无统计学意义。结论携带HBV保护性抗原基因的重组MVA病毒可以诱导针对抗原的特异性细胞免疫和体液免疫,将其与表达相同抗原的DNA疫苗联合,采用DNAprime/rMVA boost的免疫方案,可以诱导比两者单独应用更强的细胞免疫反应。IL-18对免疫效果无明显影响。Objective To investigate the effect of different immune progress on the immune efficacy of DNA vaccine expressing HBV PreS2 - S gene. Methods C57BL/6 mice were immunized by different immunization processes, including single DNA vaccine immunization, single rMVA immunization, DNA prime/rMVA boost and DNA prime/rMVA boost ＋ mIL - 18. The effects of humoral immunity and cellular immunity were evaluated by ELISA and ELISPOT. Results There was no statistical difference in antibody generation time and titer among the vaccine groups capable of expressing two antigens. The ELISPOT detection of IFN- γ shewed that all immunization groups could induce cellular immunity against the specific antigen. The effect of the single rMVA immunization group was better than that of the single DNA vaccine immunization group, lout weaker than that of the DNA prime/rMVA boost group and the DNA prime/rMVA boost ＋ mIL- 18 group. There was no statistically significant difference between the DNA prirne/rMVA boost group and the DNA prime/rMVA boost ＋ mIL- 18 group. Conclusions The recombinant MVA virus expressing HBV PreS2 - S gene induced specific cellular and humoral immune response toward HBV antigen. With the strategy of DNA prime/rMVA boost, stronger cellular immune is induced than that of DNA vaccine or rMVA alone, which provides the basic evidence for the application of gene therapy for HBV.

关 键 词：乙型肝炎病毒 DNA疫苗 免疫效果 

分 类 号：R392-33[医药卫生—免疫学]