HPLC测定复方补骨脂缓释片中葛根素的含量  

DETERMINATION OF PUERARIN IN FUFANG-BUGUZHI SUSTAINED RELEASE TABLETS BY HPLC

作　　者：蒲清荣[1] 赵剑[1] 杨荣平[2] 陈玉兰[1] 颜章龙[1] 

机构地区：[1]泸州医学院附属中医医院,泸州646000 [2]重庆市中药研究院

出　　处：《现代预防医学》2010年第1期103-104,116,共3页Modern Preventive Medicine

摘　　要：[目的]建立复方补骨脂缓释片中葛根素的含量测定方法。[方法]采用高效液相色谱法。色谱柱:SU-PELCO Discovery C18柱;流动相:乙腈(A)-0.1%乙酸水溶液(B)进行二元梯度洗脱;流速:1ml/min;波长:250nm;柱温:30℃。[结果]葛根素在0.1188～1.4860μg之间线性关系良好,其回归方程为:A=4E+06C-84328r=1;回收率在98%～104%之间。[结论]该方法准确、可靠、重现性好,可以用于复方补骨脂缓释片的质量控制。[Objective] To determine Puerarin in Fufang-Buguzhi Sustained Release Tablets. [Methods] HPLC method was used. Solid phase：SUPELCO Discovery C18 column; liquid phase：CH3CN and 0.1% acetic acid duality grads; flow rate：1 ml/ min; wavelength：250 nm; temperature：30℃. [Results] The calibration curve of HPLC was very good linear in the range of 0.1188-1.4860μg,and the regression equation was A=4E＋06C-84328 r=1,recovery rate was within 98%-104%. [Conclusion] This method is convenient,reliable,and can be used to control the quality of Fufang-Buguzhi Sustained Release Tablets.

关 键 词：HPLC 复方补骨脂缓释片 葛根素 

分 类 号：R927.2[医药卫生—药学]