大鼠海马结构内有髓神经纤维老年性改变的体视学研究  

Stereological study of the age-related changes of the myelinated nerve fibers in the hippocampal formation of rat

作　　者：卢伟[1] 杨姝[1] 李琛[1] 师晓燕[1] 陈林[1] 徐瑲[2] 张伟[2] 唐勇[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科 [2]重庆医科大学组织学胚胎学教研室,重庆400016

出　　处：《解剖学报》2009年第6期851-856,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(30440082;30572075);国家人事部2005年度高层次留学人才回国工作资助经费;教育部重点科研项目;教育部留学人员科研启动基金;重庆市自然科学基金(CSTC;2005BB5034)资助

摘　　要：目的探讨雌性Long-Evans大鼠海马结构及其内有髓神经纤维的老年性改变。方法运用透射电子显微镜和体视学方法分别对5只青年(6月龄)、5只中老年(18月龄)和6只老年(28月龄)雌性Long-Evans大鼠海马结构及其内有髓神经纤维进行定量研究。结果青年组、中老年组和老年组大鼠的海马结构总体积,海马结构内有髓神经纤维的体积分数和总体积,有髓神经纤维的长度密度和有髓神经纤维平均直径均未见显著性改变。中老年组大鼠海马结构内有髓神经纤维总长度与青年组相比增加了63.6%,老年组有髓神经纤维总长度与中老年组相比下降了47.5%,老年组有髓神经纤维总长度与青年组比较下降了13.8%。结论本研究结果进一步支持正常老年大脑的有髓神经纤维存在广泛性老年改变。Objective To explore the changes of the hippocampal formation and the myelinated nerve fibers in the hippocampal formation of aged female Long-Evans rat. Methods The hippocampal formation and the myelinated nerve fibers in the hippocampal formation of 5 young （6-month old）, 5 middle-aged （18-month old） and 6 aged （28-month old） female Long- Evans rats were quantitatively investigated with the stereological techniques and transmission electronic microscope technique. Results There were no significant changes in the volume of hippocampal formation, the volume density, the total volume, the length density and the mean diameter of the myelinated nerve fibers in the hippocampal formation among young, middle-aged and aged rats. When compared to that of young rats, the total length of the myelinated nerve fibers in the hippocampal formation of middle-aged rats was significantly increased by 63.6% . When compared to that of middle-aged rats, the total length of the myelinated nerve fibers in the hippocampal formation of aged rats was significantly decreased by 47.5 % . When compared to that of young rats, the total length of the myelinated nerve fibers in the hippocampal formation of aged rats was non-significantly decreased by 13.8 %. Conclusion Although the reason why the total length of the myelinated nerve fibers in the hippocampal formation of middle-aged rats was longer than that of young rats needs further investigations, the present results together with our previous findings in white matter and cortex further suggest that there are age-related changes of the myeliuated nerve fibers in the normal aged brains.

关 键 词：海马结构 有髓神经纤维 老年改变 体视学 大鼠 

分 类 号：R329.54[医药卫生—人体解剖和组织胚胎学]