海洛因、麻黄素对小鼠仔鼠下丘脑、海马组织结构及胆碱乙酰基转移酶活性的影响  

Effects of heroin and ephedrine on the histological structure and ChAT activity of hypothalamus and hippocampus of filial mice

作　　者：李重阳 高先军[2] 王昱[2] 王悦[2] 俞诗源[2] 冯红丽[2] 胡卓美[2] 郭婷婷[2] 

机构地区：[1]甘肃省政法学院公安分院,兰州730070 [2]西北师范大学生命科学学院,兰州730070

出　　处：《解剖学报》2009年第6期862-868,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(30370163);甘肃省自然科学基金(ZS031-A25-005-Z);甘肃省创新人才基金(ZS006-016)资助

摘　　要：目的探讨海洛因和麻黄素对仔鼠下丘脑、海马组织结构及胆碱乙酰基转移酶(ChAT)活性的影响,观察Bax蛋白和角质细胞生长因子(KGF)在下丘脑、海马的表达。方法108只昆明小鼠仔鼠,采用递增剂量连续腹腔注射海洛因和麻黄素,利用吉姆萨染色及免疫组织化学方法观察下丘脑和海马凋亡细胞的变化及Bax蛋白和KGF的表达,利用比色法检测下丘脑和海马ChAT活性的变化。结果仔鼠注射海洛因、麻黄素5d、10d、15d、20d,下丘脑和海马凋亡细胞及Bax蛋白和KGF阳性表达细胞的数量均高于对照组,ChAT的活性低于对照组,差异显著或极显著(P<0.05或P<0.01);海洛因组仔鼠下丘脑和海马的上述4项指标与麻黄素组相比,差异显著或极显著(P<0.05或P<0.01)。随着海洛因和麻黄素剂量的递增,仔鼠下丘脑和海马凋亡细胞及Bax蛋白和KGF阳性表达细胞的数量呈增多趋势。结论海洛因和麻黄素影响仔鼠下丘脑和海马的组织结构及ChAT活性,其机制可能与下丘脑和海马组织的细胞凋亡有一定相关性。Objective To explore effects of heroin and ephedrine on the histological structure and ChAT activity of hypothalamus and hippocampus of filial mice. Expression of Bcl-2 associated X protein（ Bax protein） and keratinocyte growth factor （KGF） of hypothalamus and hippocampus were measured. Methods One hundred and eight filial mice were given intraperitoneal injection of heroin and ephedrine by gradually increase of doses, apoptosis and expression of Bax protein and KGF of hypothalamus and hippocampus were observed by Giemsa staining and immunohistochemistry, and the ChAT activity was detected by colorimetry. Results After administration of heroin and ephedrine at 5,10, 15,20 days, the number of apoptotic cells and expression of Bax protein and KGF of hypothalamus and hippocampus were significantly increased and ChAT activity was lower than those of the control group（ P 〈 0.05 or P 〈 0.01 ）. There were differences between heroin group and the ephedrine group in the above-mentioned four indexes （ P 〈 0.05 or P 〈 0.01）. The number of apoptotic cells and Bax protein and KGF immunopositive neurons of hypothalamus and hippocampus increased by the increase in dose of heroin and ephedrine. Conclusions Heroin and ephedrine had great effect on the histological structure and ChAT activity of hypothalamus and hippocampus of filial mice, and this effects would be related to the cell apoptosis of hypothalamus and hippoeampus.

关 键 词：海洛因 麻黄素 下丘脑 海马 胆碱乙酰基转移酶 免疫组织化学 仔鼠 

分 类 号：Q954.4[生物学—动物学]