大鼠咽黏膜内ATP受体P2X_3阳性感觉纤维的分布  

Distributions of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa

作　　者：王昭金[1] 王延清 

机构地区：[1]泰山医学院解剖学教研室,山东泰安271000 [2]山东省泰山慢性病医院,山东泰安271000

出　　处：《解剖学报》2009年第6期1005-1007,共3页Acta Anatomica Sinica

基　　金：山东省卫生系统中青年重点科技人才资助项目(1020)

摘　　要：目的研究大鼠咽黏膜内三磷酸腺苷(ATP)受体P2X3阳性感觉纤维的分布,为探究ATP在咽黏膜感觉信号传导中的作用提供形态学依据。方法成年Wistar大鼠12只,应用免疫荧光组织化学双标技术结合激光扫描共焦显微镜。结果1.在咽黏膜各部位均可观察到P2X3阳性感觉纤维,纤维分为两类:一类为串珠样游离神经纤维;另一类纤维在黏膜内发出分支并形成复杂的树枝状末梢,纤维分支在黏膜内形成神经丛。2.可见许多降钙素基因相关肽(CGRP)阳性纤维缠绕在P2X3阳性树枝状末梢周围,并有少量的串珠样P2X3阳性纤维与CGRP共存。3.在岩神经节内可观察到许多P2X3或CGRP免疫阳性神经元,其中有少量神经元同时呈P2X3/CGRP免疫阳性。结论大鼠咽黏膜内不同类型的感觉纤维均表达ATP受体P2X3阳性,提示ATP可能与咽黏膜伤害性刺激和其他生理性刺激向中枢的传递有关。Objective To study the distribution of purinergic P2X3 receptor immunoreacfive afferent fibers in the rat pharyngeal mucosa and provide morphological evidence for exploring the role of ATP in the signal transduetion of sensory stimuli. Methods Twelve adult Wistar rats were used and imnmnofluorescenthistoehemieal double-labeling technique combined laser confocal scanning microscope was applied in the present study. Results 1 .P2X3 immunoreactive fibers were observed on sections from all parts of pharyngeal mueosa. Two types of positive fibers were found. One was free nerve fibers covered with many varicosifies. Another one ramified in mucosa and showed complex arborization endings. Nerve plexus in mucosa were formed by P＇2X3 immunoreactive fiber ramifications. 2. Most caleitonin gene-related peptide （CGRP） positive fibers intermingled with P2X3 immunoreactive fiber arborizafions. A few P2X1immunoreactive fibers were covered with many varicosities co-localized with CGRP. 3. In petrosal ganglion, most neurons were stained with P2X3 or CGRP immunoreaetivity and small number of P2X3 immunoreactive neurons co-stained with CGRP. Conclusion These results indicated thai the different types of afferent fibers in rat pharyngeal mucosa expressed purinergic P2X3 receptor immunoreactivity and ATP might be related to nociceptive or physiological signals transduction in rat phayngeal mueosa.

关 键 词：ATP受体 降钙素基因相关肽 感觉纤维 咽黏膜 免疫荧光组织化学 大鼠 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]