不同能流密度体外冲击波对骨质疏松兔股骨髁部松质骨影响的实验研究  

Study on effect of different energy flux density extracorporeal shock wave on femoral condyle trabecular bone in osteoporosis rabbit model

作　　者：张堃[1] 韩娟[1] 刘建[2] 孟国林[2] 袁志[2] 张钦[2] 梁国穗[3] 秦岭[3] 

机构地区：[1]甘肃省中医药研究院,兰州730050 [2]第四军医大学西京医院全军骨科研究所 [3]香港中文大学

出　　处：《中国骨质疏松杂志》2009年第12期871-876,共6页Chinese Journal of Osteoporosis

基　　金：陕西省国际科技合作重点项目(2005KW-14)

摘　　要：目的观察不同能流密度体外冲击波对骨质疏松兔股骨髁部松质骨成骨作用的差异。方法采用卵巢切除法(OVX)对30只5月龄雌性新西兰兔去势,5个月后建立骨质疏松模型。将所有骨质疏松兔随机分为3组,每组10只,其中一组为空白对照组(A组),一组为体外冲击波(ESW)能流密度0.28mJ/mm2处理组(B组),另一组为体外冲击波能流密度0.47mJ/mm2处理组(C组),脉冲次数均为2000次,在实验组兔右侧股骨髁部进行体外冲击波(ESW)处理。于处理后4、8周时分二批每组处死5只动物,分离右侧股骨远端,进行显微CT(micro-CT)测量分析。结果micro-CT三维重建分析表明,不同能流密度冲击波处理后4周时B组和C组在骨组织体积比(BV/TV)、骨小梁数目(Tb.N)等指标之间的差异有统计学意义;8周时B组和C组分别在骨密度(BMD)、骨矿含量(BMC)和BV/TV、骨表面积体积比(BS/BV)、骨小梁连接密度(Connectivity Density)等指标之间的差别有统计学意义,其中C组的BMD和BMC分别较B组增高16.8%和10.6%,尽管C组在其他骨小梁立体测量学等指标方面均较B组优,但两组测量结果的差别无统计学意义。结论不同能流密度体外冲击波对骨质疏松局部治疗后,不同时间,其成骨作用不同,较高能流密度的体外冲击波在治疗后8周时,促进骨质疏松被处理局部骨小梁的改建、改善骨小梁的三维结构、增加骨密度作用较优。Objective To investigate the difference of bone fi）rmation effects of different energy flux density extracorporeal shock wave on femoral condyle in osteoporosis rabbit in vivo. Methods Thirty New Zealand white rabbits were ovariectomied for 5 months to establish osteoporosis animal model. All animals were randomized into 3 groups： group A （sham）, group B were used 0.28 nd/mm^2 energy flux density extracorporeal shock wave and group C were used 0.47 m J/mm^2 , shock wave pulses are 2000. The right femoral condyles of all the test animal were applied. All animals were sacrificed after ESW treatment 4 or 8 weeks, and underwent micro-CT scan. Results Micro-CT analysis showed that bone volume/tissue volume（BV/TV） and trabecular number（Tb.N） have significant differences on 4 weeks after ESW treatment between the group B and C. On 8 weeks after ESW treatment, the group C, bone mineral density （BMD）, bone mineral content （BMC）, bone volume/tissue volume （BV/TV）, bone surface/tissue volume （BS/TV） and trabecular connectivity density were higher significantly than those in group B, the BMD and BMC in group C were 16.8% and 10.6% higher than those in group B respectively. Although the other micro-architectural parameters in group C were higher than those in group B on 8 weeks after ESW treatment, but no significant differences were detected between the two groups. Conclusion In different time, different energy flux density extracorporeal shock wave used on osteoporosis animal made different effect. It is showed that higher energy flux density extracorporeal shock improve the reconstruction, remodeling of cancellous bone and increase the BMD is better than others on 8 weeks.

关 键 词：骨质疏松 体外冲击波 显微CT 兔 

分 类 号：R68[医药卫生—骨科学]