子痫前期患者胎盘组织中miRNA-152和miRNA-377的表达  

Expression of miRNA-152 and miRNA-377 in human placentas of patients with preeclampsia

作　　者：俞凌[1] 朱晓明[2] 王珺[2] 姚元庆[1] 

机构地区：[1]中国人民解放军总医院妇产科,北京100853 [2]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《生殖医学杂志》2009年第6期531-534,共4页Journal of Reproductive Medicine

摘　　要：目的探讨微小RNA(microRNA,miRNA)在子痫前期患者和正常产妇胎盘组织中的表达差异,及其在子痫发病中的作用。方法收集子痫前期患者和正常产妇胎盘组织并提取RNA;采用miRNA特异引物进行反转录;荧光实时定量聚合酶链反应(real-time PCR)检测miRNA-152(miR-152)和miR-377基因的表达量。结果在子痫前期患者胎盘组织中miR-152表达升高,miR-377表达降低。结论子痫前期患者胎盘组织中存在特异miRNA的差异表达,其发病可能受miRNA的调控。Objective： To detect the expression of microRNA-152 and microRNA-377 in human placentas of patients with preeclampsia, in order to investigate the role of small RNA （microRNA, miRNA） in the pathogenesis of preeclampsia. Methods： Placentas were obtained from patients with preeclampsia and normal parturients; Looped miRNA specific RT primers were used to reversely transcribe the miRNA, miRNA were then amplified by SYBR Green real-time RCR using miRNA specific PCR primers. Results： The expression of miRNA 152 in the human placentas of patients with preeclampsia was elevated, that of miRNA-377 was decreased. Conclusions： The present study suggests that the specific miRNA are distinctive expression in the human placentas of patients with preeclampsia, specific miRNA may involve in preeclampsia occurrence.

关 键 词：MIRNA 子痫 先兆 胎盘 患者 PCR引物 小分子RNA 微RNA 

分 类 号：Q522[生物学—生物化学] S853.51[农业科学—临床兽医学]