沼气高产菌群保藏条件的比较  

Comparison of Biogas Flora Preserved Condition

作　　者：沙长青[1] 谷军[1] 原韬[1] 张晓彦[1] 于德水[1] 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010

出　　处：《生物技术》2009年第6期55-57,共3页Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2007AA100705)资助

摘　　要：目的:利用不同方法对沼气高产菌群进行保藏,比较各方法不同时间的保藏效果。方法:应用液体低温冷藏法、液体石蜡封存法、液氮冷冻保藏法和低温冷冻干燥保藏法保藏的菌种,分别在保藏后1个月、3个月、6个月及1年后复苏菌种,测定其产气速度、产气量。结果:以上几种方法均能够保证所保藏菌群在1个月内得到复苏并产气,低温冷冻干燥法及液氮冷冻法保藏沼气产生菌菌群可达1年以上,产气速度、产气量较为理想,优于液体低温冷藏法及液体石蜡法。Objective： Compariod different preservation methods of biogas yield flora. Method： Liquid low - temperature freezing method, paraff in wax preservation, liquid nitrogen freezing preservation and the low- temperature freeze- drying preservation preserved strains were tested. After one month, three months, six months and one year the recovery gas production were compared. Result： Those methods were more than able to guarantee the flora preserved within one month. Low - temperature freeze - drying method and liquid nitrogen freezing preservation of biogas flora can last for up to one year, gas production rate and gas producing performance is much better than those of the previous methods.

关 键 词：沼气 菌群 保藏方法 

分 类 号：Q93-336[生物学—微生物学]