沙利度胺联合曲安奈德抑制人脐静脉内皮细胞增殖的研究  

The Effect of Thalidomide and Triamcinolone Acetonide on Human Umbilical Vein Endothelial Cells

作　　者：马敏旺[1] 陈松[2] 李文博[2] 

机构地区：[1]武警医学院附属医院眼科,300162 [2]天津市眼科医院

出　　处：《天津医药》2009年第12期1035-1037,I0007,共4页Tianjin Medical Journal

基　　金：天津市科技发展计划资助项目(项目编号:33111411)

摘　　要：目的:研究沙利度胺(thalidomide)联合曲安奈德对人脐静脉内皮细胞(HUVEC)的药物作用。方法:Ⅰ型胶原酶消化法原代培养HUVEC,Ⅷ因子相关抗原检测法鉴定细胞。沙利度胺溶于0.5%二甲基亚砜(DMSO)中,分为3组。A组为沙利度胺组,分4个浓度梯度,分别为10、25、50和75mg/L;B组为沙利度胺+曲安奈德组,为含1mg/L曲安奈德的各浓度沙利度胺;C组为对照组。HUVEC传代植入96孔板,细胞密度为1×104/mL,过夜,加入不同浓度的药物,处理72h;在酶标仪570nm波长处读取吸光度(A)值。结果:2组药物均对HUVEC的增殖有明显的抑制作用(P<0.01),抑制作用与药物浓度呈正相关;与A组相比,B组对HUVEC抑制作用更强(P<0.05)。结论:沙利度胺及曲安奈德对血管内皮细胞增殖有明显作用,这两类药物在治疗新生血管性疾病中可发挥一定作用。Objective： To investigate the effect of thalidomide and triamcinolone acetonide （TA） on human umbilical vein endothelial cells （HUVEC）. Methods： The primary culture of HUVEC with collagenase Ⅰ was identified with FⅧAg. There were 3 groups in the study including thalidomide group （10, 25, 50 and 75 mg/L）, TA and thalidomide group and control group. The absorbance ratio was measured through MTT-test. Results： Both thalidomide group and TA and thalidomide group showed inhibitor effect on the growth of HUVEC significantly （P 0.01）. The inhibitory effect was dose dependent. Compared to thalidomide group, the inhibitory effect was more significant in TA and thalidomide group （P 0.05）. Conclusion： Thalidomide can inhibit the growth of HUVEC; however, the effect was more significant in TA and thalidomide group.

关 键 词：内皮 血管 脐静脉 曲安奈德 沙立度胺 细胞 培养的 

分 类 号：R285.5[医药卫生—中药学] R733.3[医药卫生—中医学]