中国人感染H5N1亚型高致病性禽流感病例病毒分离培养探讨  

The virus isolation analysis of the H5N1 subtype human avain influenza cases in China's Mainland from 2005 to 2009

作　　者：邹淑梅[1] 张烨[1] 李梓[1] 董婕[1] 赵新生[1] 董丽波[1] 温乐英[1] 徐翠玲[1] 王敏[1] 郭俊峰[1] 隗合江[1] 高荣宝[1] 王秀平[1] 舒跃龙[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2009年第6期409-411,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的分析中国大陆人感染H5N1高致病性禽流感病例标本采集和病毒分离培养之间的关系。方法对2005年10月至2009年3月中国大陆人感染H5N1高致病性禽流感病例标本进行病毒分离培养。结果标本采集时间主要集中在发病后的0-14d，其中0-7d采样的标本阳性率分布相对集中，病毒分离的效果要更加显著；标本采集类型主要为呼吸道标本，其中下呼吸道标本的病毒分离阳性率分布较集中，病毒分离效果也更明显。结论人禽流感病例标本采集时间及标本的采集类型对病毒分离有一定的影响，应在发病的急性期侧重于采集下呼吸道标本，这样利于提高病毒分离的阳性率。Objective To analyse the correlation between the virus isolation and the specimen collection of the H5N1 human high pathogenic avain influenza cases in China's Mainland. Methods The specimens were collected in China's Mainland from 2005.10 to 2009.3 and the H5N1 viruses were isolated by passage in embryonated chicken eggs. Results Most specimens were obtained within 14 days after disease onset. For the specimens collected within 7 days, the isolation rate was relatively high and the difference of the positive rate between different years was lower than those specimens collected after 7 days. Most of the samples in our study were collected from the upper or lower respiratory tract with few from blood, feces, et al. The isolation rate of lower respiratory specimens was higher and the difference of the positive rate between different years was relatively lower than those from upper respiratory specimens. Conclusion We suggest that the samples should be collected from lower respiratory tract during the acute phase to get the higher isolation rate.

关 键 词：流感病毒A型 鸟 病毒培养 选择偏倚 

分 类 号：R51[医药卫生—内科学]