表达HIV-1 gag基因的重组AAV2／1和Ad5疫苗免疫原性比较  

Comparison of the immunogenicity of rAAV2/1 and tad5 expressing HIV-1 gag

作　　者：余双庆[1] 冯霞[1] 刘红梅[1] 杨海儒[1] 李红霞[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2009年第6期421-423,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重大科技专项（2008ZX10001-009）;国家自然科学基金（30872397）.

摘　　要：目的在小鼠体内比较表达HIV-1 gag基因的重组AAV2／1和Ad5疫苗的免疫原性。方法分别用rAAV2／1-gag或rAd5-gag单独免疫BALB／c小鼠一次或两次，于免疫后不同时间点用CFSE染色法和胞内细胞因子染色法检测Gag特异性细胞免疫应答，用ELISA方法检测Gag特异性抗体反应。结果单次免疫结果显示，在所有检测点tAd5-gag所诱导的Gag特异性CTL应答都比rAAV2／1-gag强，抗体反应在免疫后4周无明显差异。两次免疫后4周的检测结果表明，rAd5-gag所诱导的Gag特异性CTE应答比rAAV2／1-gag强，但抗体反应比rAAV2／1-gag组弱。结论rAd5-gag诱导了很好的HIV-1特异性细胞和抗体反应，而rAAV2／1-gag诱导的HIV-1特异性抗体反应很强，但细胞免疫应答较弱。Objective To compare the immunogenicity of rAAV2/1 and rAd5 expressing HIV-1 gag in BALB/c mice. Methods BALB/c mice were immunized with rAAV2/1-gag or rAd5-gag once or twice. HIV-1 specific cellular immune responses were analyzed by in vivo CTL and intracellular cytokine staining assays. HIV-1 Gag specific antibodies were tested by ELISA. Results Mice immunized with rAdS-gag once induced stronger Gag specific cellular immune responses and similar level of Gag specific antibody compared with rAAV2/1-gag. Mice immunized with rAd5-gag reached the peak immune responses more rapidly than rAAV2/1-gag. However, mice immunized with rAAV2/1-gag twice elicited better Gag specific IgG. Conclusion rAd5-gag induced strong HIV-1 specific cellular and antibody responses, and rAAV2/1-gag induced high level of HIV-1 specific IgG and moderate cellular immune responses.

关 键 词：HIV-1 细小病毒科 腺病毒科 艾滋病疫苗 

分 类 号：R392[医药卫生—免疫学]