抗-HBs定量方法的改进  

Improvement of quantitative method on anti-HBs

作　　者：王锋[1] 余陶[1] 张文英[1] 张勇[1] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《中华实验和临床病毒学杂志》2009年第6期485-487,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的通过对系列已知浓度标准品的检测来间接进行血样抗-HBs的定量，比较两种计算方法的准确程度，从而选出适合本实验室的抗-HBs定量方法。方法用RIA法检测抗.HBs系列已知浓度标准品，将测定结果与对应的浓度分别用log-log模型和指数曲线模型拟合得到标准曲线方程，比较两种曲线拟合的效果。再将对标定浓度质控品的测定值分别代入上述两种模型的曲线方程计算出质控品的浓度，比较两种计算方法定量结果的准确程度。结果指数曲线模型的误差均方较小，为1.2971；而且确定系数R^2为0.9904，接近1。通过两种曲线方程计算的标定浓度（30.0mlU／ml）、质控品平均浓度（n=6）分别为（32.28±1.06）和（31.91±1.06）mlU／ml，用指数曲线模型计算的浓度与标定浓度相比仅偏高6.37％。结论用指数曲线模型拟合能够更好地估算待检样品的实际浓度，可作为本实验室抗-HBs的一种改进定量方法。Objective Through detecting the standard preparation with series of concentration to indirectly calculate the anti-HBs concentration of the serum samples, a suitable anti-HBs quantitative method for our laboratory was found after comparing the two kinds of methods. Methods Detecting the anti-HBs standard preparation with series of concentration by RIA method, standard curvilinear equations were obtained by the means of fitting the detected result and the corresponding concentration by log-log model and exponential curve model respectively. Then the fitting efficiency of two curves was compared. By calculating the concentrations of the reference using two standard curvilinear equations, we can compare the accuracy of two quantitative methods. Result The error mean square of the exponential curve model is low as 1.2971 and the determinate coefficient is close to 1 with the value of 0.9904. The average concentrations（ n = 6） of the detected reference calculated by two curvilinear equation with the actual concentration of 30.0mIU/mL are（32.28±1.06） and （31.91±1.06） mIU/ mL respectively. The concentration calculated by exponential curve model is only 6.37 % higher than the actual concentration. Conclusion Fitting by exponential curve model is more practical to estimate the actual concentration of the serum samples those will be detected. It can be used as an optimal quantitative method to detect anti-HBs concentration.

关 键 词：参考标准 肝炎抗体 乙型 曲线拟合 定量方法 

分 类 号：R446.6[医药卫生—诊断学]