实时RT-PCR检测159例手足口病患儿样本中肠道病毒的结果分析  

Detection and analysis of the enterovirus infection within 159 cases of Hand-foot-mouth disease by a real-time reverse transcription-PCR assay

作　　者：伏瑾[1] 张艳玲[2] 孙春荣[2] 刘燕燕[2] 崔小岱[2] 王天有[2] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]首都儿科研究所

出　　处：《中华实验和临床病毒学杂志》2009年第6期488-490,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：十一五国家科技支撑计划（2008BA170B02）.

摘　　要：目的了解2009年4-8月首都儿科研究所附属儿童医院手足口病患儿肠道病毒的感染状况，为临床诊治提供参考。方法采集首诊手足口病159例患儿的咽拭子和疱疹液标本，以肠道病毒（EV）通用型、柯萨奇病毒A16（CA16）型、肠道病毒71（EV71）型核酸检测试剂盒，应用实时RT-PCR法检测标本中的肠道病毒。选取阳性标本扩增VP1区，产物进行序列测定和分析。结果（1）EV、CA16、EV71的阳性病例数分别为152、102、43；阳性率为95.6％、64.2％、27.0％。（2）CA16占EV阳性的67.3％，EV71占EV阳性的28.3％，非CA16和EV71的EV病例7例，占EV阳性的4.6％。CA16：EV71为2.37：1。（3）部分阳性标本经测序验证与此法结果一致。结论2009年我院手足口病患儿以EV71和CA16感染为主，EV71感染的手足口病比例较2007年出现明显上升。Objective To reveal the enterovirus infection within children suffering hand-foot-mouth disease（HFMD） in the Capital Institute of Pediatrics from Aprial to August, 2009, for the sake of clinical diagnosis and treatment. Methods Both throat swab and vesicle fluid were taken respectively from 159 children with HFMD. And RNA were extracted from each sample followed with real-time fluorescence quantitative RT-PCR kits with three reagents： universal enterovirus primer, Coxsackievirus A16（CA16） primer and enterovirus 71 （EV71） primer. Parts of postivive samples were sequenced and analyzed. Results（1） EV genes were detected from 152 cases, of which, 102 cases were positive for CA16 and 43 were positive for EV71. （2） CV16：EV71 was 2.37： 1. The positive rates of throat swabs and vesicle fluid samples were not statistically significant.（3） The PCR results were same with that of sequence analysis. Conclusion The hand-foot-mouth disease recently appeared in our hospital was mainly related to the EV71 or CA16 infection. And the percentage of EV71 infectious obviously increased compared to that of 2007.

关 键 词：逆转录聚合酶链反应 肠道病毒属 手足口病 柯萨奇病毒感染 

分 类 号：R72[医药卫生—儿科]