人DNAJB6蛋白与人巨细胞病毒皮层蛋白pUL23相互作用的鉴定  

Identification of Human DNAJB6 as an Interacting Protein for HCMV Tegument Protein pUL23

作　　者：姚伙旺[1] 李实骞[1] 胡嘉淼[1] 陈业志[1] 邹奕[1] 张欣[1] 李月琴[1] 周天鸿[1] 李弘剑[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程学系,广州510632

出　　处：《中国生物化学与分子生物学报》2009年第12期1143-1148,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.30770106);国家自然科学基金重大研究计划面上项目(No.90608024);广东省自然科学基金项目(No.06025162)~~

摘　　要：pUL23是人巨细胞病毒(HCMV)UL23基因编码的皮层蛋白.HCMV皮层蛋白与病毒颗粒的形成、病毒转移、免疫调控等病毒生活过程相关.利用GAL4酵母双杂交系统筛选人胚肾cDNA文库,获得与人巨细胞病毒皮层蛋白pUL23相互作用的宿主蛋白分子DNAJB6[DnaJ(Hsp40)homolog,subfamily B,member 6].回复酵母双杂交、体外GST-Pull down和免疫共沉淀试验再次确认两者之间的相互作用.该结果为进一步研究pUL23蛋白在HCMV生活周期中的作用机制提供依据.Human cytomegalovirus（HCMV） is an opportunistic human pathogen that can cause life-threatening diseases in immunologically immature or compromised individuals,including neonates,AIDS patients and transplant recipients.UL23 gene encodes a tegument protein（pUL23） about 33 kD,of which the role has been implicated in the formation,transport,immunomodulation and transactivation of HCMV.To investigate the underlying function of UL23 during HCMV infections,we screened a human fetal kidney cDNA library using the GAL4 yeast two-hybrid assay.One of the positive clones was identified as the DNAJB6 [DnaJ（Hsp40） homolog,subfamily B,member 6].The interaction between DNAJB6 and pUL23 was further confirmed by GST-pull down and co-immunoprecipitation（Co-IP） experiment with cos-7 cells co-transfected with two plasmids containing DNAJB6 and pUL23.These results may provide important information to the understanding of pUL23 in the HCMV life cycle.

关 键 词：皮层蛋白pUL23 人巨细胞病毒 酵母双杂交 蛋白质相互作用 DNAJB6 

分 类 号：Q93[生物学—微生物学] Q25