阿司匹林抑制内皮素-1诱导的大鼠心脏成纤维细胞增殖  

Aspirin inhibits the proliferation of rat cardiac fibroblasts induced by endothelin-1

作　　者：张志国[1] 杨力[2] 郑亚萍[1] 赵艳峰[3] 

机构地区：[1]漯河医学高等专科学校生理教研室,湖北武汉430071 [2]漯河医学高等专科学校免疫学与病原生物学教研室,河南漯河462002 [3]武汉大学生理教研室,湖北武汉430071

出　　处：《基础医学与临床》2009年第12期1310-1313,共4页Basic and Clinical Medicine

摘　　要：目的探讨阿司匹林（aspirin）对内皮素-1（ET-1）诱导的大鼠心脏成纤维细胞（CFs）增殖的干预作用及可能机制。方法经差速贴壁法培养的新生大鼠CFs,随机分为7组：对照组、ET-1组、阿司匹林组、ET-1＋阿司匹林1、2、5和10 mmol/L组。用四氮唑盐（MTT）法测定CFs数目,流式细胞仪检测CFs细胞周期,液体闪烁计数仪测定CFs[^3H]-脯氨酸掺入率,硝酸还原酶法测定细胞培养上清液中NO含量。结果与对照组相比,10^-7mol/L ET-1能显著促CFs增殖及[^3H]-脯氨酸掺入率,降低CFs生成NO2^-/NO3^-的量（均P〈0.01）,110 mmol/L阿司匹林呈浓度依赖性地缓解上述变化（均P〈0.05）;ET-1能显著提高S期细胞百分率（P〈0.01）,10 mmol/L阿司匹林抑制ET-1诱导S期细胞百分率上升（P〈0.01）。结论阿司匹林抑制ET-1诱导的CFs增殖及胶原合成,可能和NO生成有关。Objective To investigate the effect of aspirin on endothelin-1 induced proliferation of cardiac fibroblasts（CFs）.Methods Isolated and cultured CFs from neonatal Sprague-Dawley rats（SD） were randomly divided into 7 groups： control,ET-1,aspirin,ET-1＋aspirin 1 mmol/L,ET-1＋aspirin 2 mmol/L,ET-1＋aspirin 5 mmol/L and ET-1＋aspirin1 10 mmol/L.CFs were counted by MTT assay.Cell cycle distribution was determined with a flow cytometer（FCM）.[^3H]-Proline incorporation was evaluated by scintillation counting.Nitric oxide（NO） was measured by colorimetry.Results 10^-7mol/L ET-1 significantly increased the number of CFs and [^3H]-Pro incorporation and decreased NO2^-/NO3^-secretion compared with the control group（P〈0.01）.1～10 mmol/L Aspirin inhibited the effects of ET-1 on CFs in a concentration-dependent manner（P〈0.05 vs ET-1）.In the ET-1 group,the percentage of cells in the S phase was higher than that of the control,which was inhibited by 10 mmol/L Aspirin（P〈0.01 vs ET-1 and control）.Conclusion Aspirin can restrain the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1,and this effect may be partially mediated by NO.

关 键 词：内皮素-1 心脏成纤维细胞 阿司匹林 缓激肽 一氧化氮 

分 类 号：R541[医药卫生—心血管疾病]