水分胁迫下毛竹幼苗光合及叶绿素荧光特性的响应  

Photosynthetic and chlorophyll fluorescent responses of Phyllostachys pubescens seedlings to water deficiency stress

作　　者：应叶青[1] 郭璟[1] 魏建芬[1] 邹奕巧[1] 胡冬春[1] 方伟[1] 

机构地区：[1]浙江林学院浙江省现代森林培育技术重点实验室

出　　处：《北京林业大学学报》2009年第6期128-133,共6页Journal of Beijing Forestry University

基　　金：浙江林学院研究生创新基金重点项目(2112008001)

摘　　要：为分析水分胁迫对毛竹幼苗光合、荧光特性及水分利用效率的影响,以1年生毛竹为试验材料,采用气体交换和叶绿素荧光成像技术,研究不同水分亏缺条件下毛竹幼苗叶片光合生理参数的变化。研究表明:①随着胁迫程度的加深,光系统PSⅡ反应中心活性减弱与光合速率降低一致,气孔限制及PSⅡ反应中心可逆失活或破坏是其净光合速率(Pn)降低的主要原因;②从无胁迫(NS)处理至重度胁迫(HS)处理,光饱和点(LSP)下降20.45%,最大净光合速率(Pnmax)下降67.22%、PSⅡ最大光化学量子产量(Fv/Fm)减少22.46%,有效荧光产量减少45.60%;③低度胁迫(LS)处理水分利用效率较NS处理提高17.81%,光化学淬灭系数(qP)和非光化学淬灭系数(qN)先降低后升高;④初始荧光(Fo)、最大荧光(Fm)、Fv/Fm变异系数均随胁迫处理程度加深而增大,Fo变异区间为18.182%～26.404%,Fm为16.321%～24.899%,Fv/Fm为1.291%～33.932%。With leaves of 1-year-old Phyllostachys pubescens seedlings under water deficiency to different extents,changes in photosynthetic parameters were studied using a gas exchange photosynthesis system,Mini-Pam chlorophyll fluorometer and fluorescence imaging in order to understand the effects of water deficiency stress on photosynthetic and chlorophyll fluorescent characteristics and water use efficiency. Results showed that：1） with an increase of water deficiency stress,decrease in activity of the photosystem II （PSII） central reaction was consistent with the decreasing photosynthetic rate,which mainly resulted from stomatic limitation and inactivation or damage to PSII central reaction reversibility; 2） from non-stress （NS） to heavy stress （HS）,light saturation points（LSP）,the maximum net photosynthetic rate,Fv/Fm and effective quantum yield decreased by 20.45%,67.22%,22.46% and 45.60%,respectively; 3） water use efficiency （WUE） increased by 17.81% under light stress than under NS,while photochemical quenching coefficient （qP） and non-photochemical quenching coefficient （qN）decreased first,followed by an increase then; and 4） variation coefficients of Fo,Fm and Fv/Fm increased with the increased water deficiency stress,the values of which ranged from 18.182% to 26.404%,from 16.321% to 24.899% and from 1.291% to 33.932%,respectively.

关 键 词：毛竹 水分亏缺胁迫 光合作用 叶绿素荧光 

分 类 号：S795.7[农业科学—林木遗传育种] S718.43[农业科学—林学]