脱细胞骨微管用于构建组织工程化听骨赝复物的可行性  

Possibility of the acellular bony microtubule used to construct tissue engineering ossicular prosthesis

作　　者：李雪盛[1] 孙建军[1] 徐成[2] 

机构地区：[1]海军总医院全军耳鼻咽喉中心,北京100048 [2]海军总医院骨科,北京100048

出　　处：《中国耳鼻咽喉头颈外科》2009年第11期611-613,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：中国博士后科学基金资助项目(20080440224)

摘　　要：目的观察脱细胞骨微管用于制备组织工程化听骨的可能性。方法制备脱细胞骨微管,观察其组织相容性及生物力学变化,并作为含复合骨形态发生蛋白2(bone morphogenetic protein,BMP-2)胶原海绵载体,植入动物中耳空腔,进行异位诱导成骨观察。结果脱细胞骨微管具有良好的生物相容性,其生物力学性能无显著变化。负载含BMP-2胶原海绵后,可以在中耳腔内诱导形成新生骨组织。结论脱细胞骨微管负载含BMP-2胶原海绵可用于构建组织工程化听骨赝复物。OBJECTIVE To study the possibility of acellular bony microtubule used to construct tissue engineering ossicular prosthesis. METHODS To prepare the acellular bony microtubule, observe the change of its histocompatibility and biomechanics. The acellular bony microtubule carried with collagen/ BMP-2 was implanted into the middle ear of the animals. The ectopic osteogenesis was observed. RESULTS The acellular bony microtubule has good histocompatibility. There wasn’t significant change about biomechanics. After carried with collagen/BMP-2, the bony microtubule can induce osteogenesis in the animal’s middle ear. CONCLUSION The acellular bony microtubule carried with collagen/BMP-2 has the possibility to be used to construct tissue engineering ossicular prosthesis.

关 键 词：组织工程 听小骨假体 骨形态发生蛋白质类 微管 

分 类 号：R764[医药卫生—耳鼻咽喉科]