大鼠死后肋软骨细胞核DNA含量随死亡时间的变化  

A postmortem semi-quantitative study on time-dependent changes of DNA content in chondrocytes in rat's costal cartilage

作　　者：邢浩伟[1,2] 陈玉川[3,2] 成建定[2] 高翠莲[3] 罗光华[4] 

机构地区：[1]广州市番禺区人民检察院,广东广州511400 [2]中山大学中山医学院法医学系,广东广州510080 [3]广东警官学院,广东广州5102302 [4]广东司法警官职业学院司法鉴定系,广东广州510520

出　　处：《中国法医学杂志》2009年第6期365-367,共3页Chinese Journal of Forensic Medicine

基　　金：广东省自然科学基金资助项目(036628;05001685)

摘　　要：目的研究大鼠死后肋软骨细胞核DNA含量与晚期死亡时间的相关性。方法应用改良的Feu lgen法结合计算机图像分析技术对死后大鼠35d内肋软骨细胞核DNA含量变化进行观测分析。结果死后1~28d,细胞核染色逐渐变淡,至35d时已观察不到,死后肋软骨细胞核DNA含量与死亡时间呈直线相关。结论大鼠肋软骨细胞核DNA含量随死亡时间延长呈线性规律下降。Objective To study the relationship between the DNA content of chondrocytes in the costal cartilage and postmortem interval in putrefactive rat cadavers. Methods Nuclear DNA was visualized by modified Feulgen＇ s staining method. DNA content of chondrocytes in the costal cartilage was semi-quantitatively determined by a computerized image analysis system in rats within 35d postmortem. Results Staining intensity of the nuclei was gradually reduced within from ld to 28d postmortem. The nuclei could not be detected at 35d. The DNA content of chondrocytes decreased time-dependently within 28 days after death as determined semi-quantitatively, which revealed a linear relationship between DNA content and postmortem interval. Conclusion DNA content of chondrocytes in the costal cartilage reduces time-dependently with the extension of postmortem interval.

关 键 词：法医病理学 死亡时间推断 DNA含量 腐败尸体 肋软骨 软骨细胞 

分 类 号：D919[医药卫生—法医学]