家兔唾液与血液中氯胺酮浓度的相关性  

Correlation with ketamine concentration in plasma and saliva in rabbits

作　　者：李鹏旺[1] 刘俊芳[1] 王玉瑾[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001

出　　处：《中国法医学杂志》2009年第6期389-391,共3页Chinese Journal of Forensic Medicine

基　　金："十一五"国家科技支撑计划项目(2007BAK26B05);山西省自然科学基金资助项目(2007011105)

摘　　要：目的研究家兔唾液中氯胺酮及代谢物去甲氯胺酮浓度与血药浓度的相关性。方法实验家兔分为氯胺酮灌胃组（6只）、静脉注射组（6只）和对照组（6只）,分别于染毒前、后不同时间点收集唾液和血液。采用气相色谱/质谱联用（GC/MS）全扫描定性、气相色谱（GC）定量分析样品中氯胺酮及去甲氯胺酮的浓度。采用双变量Pearson相关分析研究唾液中药物浓度和血药浓度的相关性。结果氯胺酮灌胃组和静脉注射组给药后各时间点氯胺酮及去甲氯胺酮在唾液和血液中的浓度相关系数（r）范围为0.80-0.95。结论氯胺酮及去甲氯胺酮在唾液和血液中的浓度均有良好的相关性,根据唾液药物浓度推断血药浓度可用于氯胺酮滥用的法医学鉴定。Objective To study the correlation with ketamine and its metabolite norketamine concentration in plasma and saliva in acute toxic rabbits. Methods Experimental rabbits were given intragastric （ i. g. group, n = 6） and intravenous （ i. v. group, n = 6） administration of ketamine respectively, and control rabbits （ n = 6 ） were given a same volume of physiologic saline. The plasma and saliva were collected before and after ketamine administration. Ketamine and norketamine in plasma and saliva were determined using GC/MS and GC. The correlation with ketamine （norketamine） concentration in plasma and saliva were analyzed by a double variable Pearson correlation analysis. Results The correlation coefficients（r） of ketamine （norketamine） concentrations in plasma and saliva were from 0.80 to 0.95 in ketamine i.g. group and i. v. group. Conclusion There is a good relativity between the concentration of ketamine （norketamine） in plasma and in saliva. The ketamine （norketamine） concentration in saliva can be used to estimate the plasma concentration in the forensic identification of ketamine abuse.

关 键 词：法医毒物学 氯胺酮 去甲氯胺酮 唾液 血浆 相关性 

分 类 号：D919.1[医药卫生—法医学]