PKC在青壮年猝死综合征心肌组织中的变化  

The variance of PKC in cardiac cells of SMDS victims

作　　者：徐宏泉 竞花兰[2] 张宜骏 闫记全 温涌溪 林泓 姜富学[4] 

机构地区：[1]深圳市人民检察院技术处,广东深圳518023 [2]中山大学中山医学院法医学系,广东广州510080 [3]广州市人民检察院技术处,广东广州510000 [4]广州市公安局刑科所,广东广州510000

出　　处：《中国法医学杂志》2009年第6期396-398,I0010,共4页Chinese Journal of Forensic Medicine

基　　金：深圳市科技局科研项目(2002-K3-117)

摘　　要：目的探讨蛋白激酶C(PKC)与青壮年猝死综合征之间的关系。方法取30例青壮年猝死综合征、20例正常对照心脏标本的左右心室肌、窦房结、房室结组织,通过免疫组化学染色,应用图像分析软件对PKC分布及强度变化进行定量检测分析,并进行统计学处理。结果青壮年猝死者心肌细胞PKC平均灰度值及面积代数和显著高于正常对照组(P<0.05);其中青壮年猝死者窦房结中的PKC平均灰度值大于房室结(P<0.05)。结论PKC作为体液调节的途径之一,参与青壮年猝死发病过程,并起到保护性作用。PKC可以作为诊断青壮年猝死的一项参考指标。Objective This article aims to explore the relationship between PKC and SMDS （sudden manhood death syndrome, SMDS）. Methods By way of immunohistochemical staining of left, right ventricular musles, sino atrial node and atrioventricular node from 30 cases of SMDS group and 20 cases of control group, we observed and measured the expression and the intensity variance of PKC in myocardial cells with graphical analysis software. Results The average gray value and total area of PKC in myocardial cells of cases in the SMS group are obviously higher than that of cases in control group （ P 〈 0.05 ）. And in the SMDS group, the average gray value of PKC in sino atrial node is higher than that in atrioventricular node. Conclusion As one way of humoral regulation, PKC is involved in the process of protecting the heart from impairment in SMDS, and it can be considerated as a reference index to identify the SMDS.

关 键 词：法医病理学 蛋白激酶C 青壮年猝死综合征 免疫组化 

分 类 号：D919[医药卫生—法医学]