国产磁珠结合自动化工作站批量提取生物检材DNA的应用  

Application of a new magnetic beads made in China in DNA extraction of forensic biological samples with automation workstation

作　　者：杨电[1,2] 刘宏[1,2] 刘超[2] 武清毓[2] 李越[2] 刘长晖[2] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515 [2]广州市刑事科学技术研究所,广东广州510030

出　　处：《中国法医学杂志》2009年第6期404-406,共3页Chinese Journal of Forensic Medicine

基　　金：公安部应用创新计划资助项目(2005YYCX57136);广东省重点科技项目(2005B33701008)

摘　　要：目的建立国产磁珠结合自动化工作站批量提取案件中生物检材DNA的方法。方法采用国产磁珠结合Bio-Robert Universal System自动化工作站对案件中常见的生物样本进行DNA提取,检测Identifiler系统16个STR基因座,在ABI3130XL遗传分析仪上进行STR分型。其中210份样品同时在ABI7500型荧光定量PCR仪上进行定量。结果9100份10类生物检材应用国产磁珠结合自动化工作站,大部分可提取到足够的DNA进行STR检验。STR检验成功率最高的为口腔拭子、肌肉,达100%,接触细胞检材的成功率较低,为50.0%。结论国产磁珠结合自动化工作站可用于案件中常见的大部分生物样本的DNA提取。Objective To study the application of a new magnetic beads made in China in DNA extraction of forensic biological samples with automation workstation . Methods DNA was extracted from common forensic biological samples by QIAGEN Bio-Robert Universal System and a new magnetic beads made in China , and then typed with Identifiler system in ABI3130XL Genetic Analyzer. 210 of these samples were also quantitated by ABI7500 Real Time System. Results Total of 9100 genomic DNA was extracted from various forensic biological samples by the new magnetic beads made in China and automation workstation methods, and most of them were successfully typed for STR analysis. In these biological samples, oral swabs and muscles were of the highest success rate of STR typing （ 100% ）, and the lowest was touched cell samples （50.0%）. Conclusion The new magnetic beads made in China with automation workstation methods can be applied to DNA extraction of most forensic biological samples.

关 键 词：法医物证学 国产磁珠 自动化工作站 DNA定量 复合STR扩增 

分 类 号：D919[医药卫生—法医学]