A型肉毒毒素对兔耳增生性瘢痕组织中P物质、β1转移生长因子、α平滑肌肌动蛋白的影响  

Effect of botulinum toxin type A injection on the expression of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar

作　　者：王琳[1] 邰宁正[1] 范志宏[1] 

机构地区：[1]上海交通大学医学院附属仁济医院整形外科,上海200127

出　　处：《中华医学美学美容杂志》2009年第6期410-413,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：基金项目：上海市科委资助项目（编号：074119606）

摘　　要：目的探讨A型肉毒毒素（botuliniumtoxintypeA，BTA）对兔耳增生性瘢痕组织中P物质（substanceP，SP）、β1转移生长因子（transforminggrowthfactorbeta一1，TGF—β1）和α平滑肌肌动蛋白（alphasmoothmusleactinA，αSMA）的影响及意义。方法12只日本大耳白兔，体重3kg，建立兔耳增生性瘢痕模型。将兔耳创面分为BTA注射组（I组）和瘢痕组（S组），每组72个创面。大体观察创面愈合时间和瘢痕增生情况，统计术后14d时的创面愈合率。术后28d时，同法另取6只兔子的兔耳腹面正常皮肤为空白对照组（C组），收集3组标本。实时定量PCR检测各组标本中SP、TGF-β1和α—SMA的mRNA含量，Western印迹法检测α—SMA的蛋白表达。结果（1）术后14d，Ⅰ组与S组的创面愈合率的差异无统计学意义；（2）1组SP和TGF一β1、α—SMA的mRNA含量较S组显著降低，但仍高于C组（P〈0．05）；（3）蛋白水平上，3组的α—sMA表达两组间比较差异均有统计学意义（P〈0．05），且S组〉Ⅰ组〉C组。结论BTA注射不延迟创面愈合，并减少了兔耳增生瘢痕中SP、TGF-β1和α—SMA的mRNA表达，为其治疗增生性瘢痕的临床应用提供了一定的理论依据。Objective To investigate the effect of botulinum toxin type A injection on the expression of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar. Methods The hyper- trophic scar model was established in 24 Japanese rabbits＇ears. The wounds in ventral surface of ear were divided in Group I （lateral wounds） and Group S （medial wounds）, 3 wounds each side per ear, totally 72 wounds each group. The wound-healing time and the growth of scar were observed and recorded. On post-wounding day 28, the wounds were created in another 6 rabbits in the same way and the normal skin were harvested as Group C. Likewise, the scar samples in Groups I and S were harvested. The mRNA expression of substance P, TGF-β1 and α-SMA were detected quantitatively by using real-time PCR and α- SMA was also detected with Western blot. Results No difference between the ratio of healed or infectious wounds on post-wounding day 14. The mRNA expression of SP, TGF-β1 and a SMA in Group I was significantly lower than Group S, but higher than those in Group C （P〈0.01 and 〈0.05, respee tively）. The blot density of α SMA and the ratio of blot density of a SMA to β-actin in Group I were between that of the other two groups. Conclusion Without delaying the wound-healing, botulinum toxin type A injection significantly decreases the expression of SP, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar.

关 键 词：A型肉毒毒素 增生性瘢痕 P物质 β1转移生长因子 Α平滑肌肌动蛋白 

分 类 号：R6[医药卫生—外科学]