厄贝沙坦对早期糖尿病肾病大鼠肾脏晚期糖基化终产物及其受体表达的影响  

Effects of irbesartan on renal advanced glycation end products and their receptor in rats with early diabetic nephropathy

作　　者：龙海波[1] 牛红心[1] 李小云[1] 周伟东[1] 何井华[1] 钟娟[1] 魏连波[1] 

机构地区：[1]南方医科大学珠江医院中西医结合肾病中心,广东广州510282

出　　处：《南方医科大学学报》2009年第12期2433-2436,2441,共5页Journal of Southern Medical University

基　　金：南方医科大学珠江医院院长基金(200403)

摘　　要：目的观察厄贝沙坦对糖尿病肾病(DN)大鼠晚期糖基化终产物(AGEs)及其受体(RAGEs)表达的影响,探讨其对DN的肾保护作用机制。方法应用链脲佐菌素建立大鼠DN模型,将DN模型大鼠随机分为2组:DN模型对照组、厄贝沙坦组;另设正常对照组。各组分别干预8周后,观察24h尿蛋白定量、血清和肾组织AGEs含量变化,免疫组化法检测肾组织RAGEs的表达,RT-PCR法检测肾组织RAGEs mRNA的表达,光镜观察肾脏病理改变。结果应用厄贝沙坦干预后,DN大鼠24h尿蛋白定量、血清及肾组织AGEs含量明显减少,肾组织RAGEs含量及其mRNA表达水平明显降低,肾脏病理改变显著减轻。结论厄贝沙坦对DN的肾保护作用机制与降低血清、肾组织AGEs水平以及肾组织RAGEs含量,抑制肾组织RAGEs mRNA表达有关。Objective To investigate the effect of irbesartan on the renal expressions of advanced glycation end products （AGEs） and their receptor （RAGEs） in rats with early diabetic nephropathy （DN） and the renoprotection mechanism of irbesartan. Methods Rat DN models established by a single injection of streptozotocin were randomly divided into the model group and irbesartan treatment group. With normal rats as the control, all the rats received daily gavage for 8 weeks. The 24-h urinary protein excretion and contents of AGEs in the serum and kidney tissues were measured. The expressions of RAGEs and RAGEs protein and mRNA in the kidney tissues were detected by immunohistochemistry and reverse transcription-polymerase chain reaction, respectively. The pathological changes of the kidney were also assessed microscopically. Results Irbesartan significantly reduced the 24-h urinary protein excretion and the contents of AGEs in the serum and kidney tissues of DN rats, resulting also in decreased expressions of RAGEs and RAGEs protein and mRNA levels in the kidney. The treatment obviously alleviated the pathological changes in the kidney of the DN rats. Conclusion Irbesartan offers renoprotection against DN possibly by reducing the serum and renal contents of AGEs and inhibiting the renal mRNA expressions of RAGEs and RAGEs.

关 键 词：糖尿病肾病 血管紧张素 厄贝沙坦 晚期糖基化终产物 晚期糖基化终产物受体 

分 类 号：R587.2[医药卫生—内分泌]