以兔红细胞作为抗原建立小鼠体液免疫反应动物模型  

Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen

作　　者：仇成凤[1] 雷林生[1] 吴玉英[1] 余传林[1] 朱正光[1] 陈娜娜[1] 吴曙光[1] 

机构地区：[1]南方医科大学药学院药物研究所,广东广州510515

出　　处：《南方医科大学学报》2009年第12期2473-2476,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(7005189)

摘　　要：目的探讨以兔红细胞作为抗原建立小鼠体液免疫反应动物模型的最适条件。方法微量溶血酶标仪分光光度法测定血清溶血素水平。结果抗兔红细胞溶血素峰时间为免疫后d6(免疫当天为d0);最适兔红细胞抗原量为20%,每鼠腹腔注射0.2ml。氢化可的松(25mg/kg)、环磷酰胺(20mg/kg)可以抑制小鼠抗兔红细胞溶血素的生成;甘露聚糖肽(4mg/kg)对正常小鼠抗兔红细胞溶血素水平影响不大,但对环磷酰胺免疫抑制小鼠的血清溶血素水平具有明显提高作用。结论以兔红细胞作为抗原建立的小鼠体液免疫反应动物模型具有实验过程简单、兔红细胞来源方便、实验误差小等优点,可用于药物对体液免疫功能影响的研究。Objective To establish a mouse model of humoral immune response by immunization with rabbit red blood cells （RRBCs）. Methods The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry. Results The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone （25 mg/kg） and cyclophosphamide （20 mg/kg） inhibited hemolysin production. Mannatide （4 mg/kg） produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide （20 mg/kg）. Conclusion Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.

关 键 词：兔红细胞 体液免疫反应 溶血素 环磷酰胺 氢化可的松 甘露聚糖肽 

分 类 号：R285.5[医药卫生—中药学]