二色补血草液泡膜H^+-ATP酶C亚基(LbVHA-C)基因克隆和表达分析  

Cloning and Expression Analysis of LbVHA-C Gene from Limonium bicolor (Bunge) Kuntze

作　　者：蒋丽丽[1] 杨传平[1] 徐晨曦[1] 马辉[1] 王玉成[1] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040

出　　处：《植物生理学通讯》2009年第12期1155-1159,共5页Plant Physiology Communications

基　　金：黑龙江省青年科技专项(QC07C56);教育部科学技术研究重点项目(107037)

摘　　要：从二色补血草cDNA文库中分离出一个V-H+-ATP酶C亚基(LbVHA-C)基因全长cDNA序列。该基因全长为729bp。其中,开放读码框(ORF)为498bp,编码165个氨基酸,预测编码蛋白的分子量为16.6kDa,理论等电点为8.62。用实时定量RT-PCR方法进一步研究二色补血草在NaCl、NaHCO3和NaCO3胁迫下的不同时间内该基因表达模式的结果表明,NaCl强烈诱导二色补血草叶组织中LbVHA-C基因的表达,但其根部的表达变化不明显。NaHCO3胁迫下LbVHA-C基因在根部组织的表达受抑制。Na2CO3胁迫24h时的LbVHA-C基因在根和叶部组织中的表达都强烈受抑制,随后在根和叶组织的表达量逐步升高。A novel vacuolar H^＋-ATPase gene （LbVHA-C） was cloned from a cDNA library of Limonium bicolor. Sequence analysis showed that the LbVHA-C gene was 729 bp in length, including 498 bp of open reading frame （ORF）, and encodes a protein of 165 amino acids with a predicted molecular mass of 16.6 kDa and pI of 8.62. The expression of LbVHA-C gene of L. bicolor in response to NaC1, NaHCO3 and Na2CO3 were investigated by real-time RT-PCR. The result showed that the expression of LbVHA-C gene was strongly induced by NaCl in leaves of L. bicolor, but not highly changed in roots. Under NaHCO3 stress, the expression of LbVHA- C gene was inhibited in roots. And the expression of LbVHA-C gene were also strongly inhibited in both roots and leaves under NaCO3 stress for 24 h, then the expression increased.

关 键 词：二色补血草 V-H+-ATP酶C亚基基因 实时定量RT-PCR 基因表达 

分 类 号：S688.4[农业科学—观赏园艺]