活性氧在苯醌致HL-60细胞增殖过程中的作用  

Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene

作　　者：刘移民[1,2] 李永胜[2] 李旭东[2] 郭晓[2] 

机构地区：[1]广州市职业病防治院,510620 [2]中山大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2009年第12期738-740,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广州市科技攻关项目（200523-E0131）;广州市医药卫生科技项目（2008-YB-114）

摘　　要：目的探讨活性氧（ROS）在苯醌（BQ）引起HL-60细胞增殖过程中的作用。方法取对数生长期的HL-60细胞，分为对照组（PBS处理细胞）、BQ染毒组（3μmol／LBQ染毒）、过氧化氢酶（CAT）＋BQ染毒组（3μmol／L BO染毒前加2000U／ml CAT），用2’-7’-二氯荧光黄双乙酯（DCFH—DA）方法检测细胞内产生ROS的量，alamar blue法检测细胞增殖率。结果BQ染毒组细胞内ROS的量（13．10±0．15）较对照组（11．32±0．09）明显增加，差异有统计学意义（P〈0．05），并且该染毒组细胞增殖率（185％±30．00％）较对照组（100％±0．00％）明显增加，差异亦有统计学意义（P〈0．05）；与BQ染毒组相比，CAT＋BQ染毒组细胞内ROS的量（10．29±0．24）和细胞增殖率（93．87％±10．00％）均明显降低，差异有统计学意义（P〈0．05）。结论在BO致HL-60细胞增殖过程中，ROS可能起着比较重要的作用。Objective To study the effect of reactive oxygen species （ROS） during the leukemogenic process associated with exposure to benzene. Methods HL-60 was treated with 3 μmol/L benzoquinone （BQ）. Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted. Results ROS production and the proliferation of cell were all increased in BQ-treated cells （13.10±0.15,185%±30.00% ） as compared with control cells （11.32±0.09,100%±0.00%） （P〈0.05） ; The addition of eatalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell （P〈0.05）. Conclusion ROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.

关 键 词：苯醌类 细胞分裂 活性氧组分 过氧化氢酶 

分 类 号：R686[医药卫生—骨科学]