碘化钾对实验性铅性肾病的保护作用及其对核因子-κB及纤维连接蛋白表达的影响  

Effect of potassium iodide on prevention of experimental lead nephropathy and expression of nuclear factor-κB and fibronectin

作　　者：乔玉峰[1] 庞东梓[3] 鹿金凤[2] 胡白瑛[4] 刘栋[4] 周桂凤[4] 杨波[4] 李荣山[1] 蒋云生[4] 

机构地区：[1]山西医科大学第二医院肾内科,太原030001 [2]山西医科大学第二医院预防保健科,太原030001 [3]山西省肿瘤医院泌尿外科 [4]中南大学湘雅二医院肾内科

出　　处：《中华劳动卫生职业病杂志》2009年第12期747-752,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的观察碘化钾对染铅大鼠肾脏组织的核因子-κB（NF-κB）及纤维连接蛋白（FN）表达的影响。方法SD大鼠72只适应性饲养1周，随机分为9组，每组8只，正常对照组，分别予蒸馏水喂饮1、2、3月；染铅组分别给予0．5％的醋酸铅喂饮1、2、3月；碘化钾组，在给予0．5％的醋酸铅喂饮的同时予碘化钾3mg／100g体重，每天1次灌胃。用电子显微镜观察肾脏超微病理改变；用免疫组化的方法检测。肾组织中NF—κB及FN蛋白的表达，用反转录-聚合酶链反应（RT—PCR）的方法检测肾组织中NF-κB及FN mRNA的表达。结果染铅3个月时，碘化钾可抑制铅所导致的肾脏小管上皮细胞和线粒体损伤。染铅1、2、3个月组肾组织中NF—κB蛋白表达分别为0．2315±0．0624、0．3213±0．0740、0．4729±0．0839，mRNA表达分别为0．4370±0．0841、0．5465±0．0503、0．6443±0．0538，较对照组明显增加，差异有统计学意义（P〈0．05，P〈0．01）。碘化钾治疗3个月组NF—κB蛋白表达为0．3152±0．0881，mRNA表达为0．5491±0．0746，较相应染铅组明显下降，差异有统计学意义（P〈0．05）。染铅2、3个月组肾组织FN蛋白表达为0．4243±0．0595、0．4917±0．0891，mRNA表达为0．8650±0．0880、0．8714±0．0980，较对照组明显增加，差异有统计学意义（P〈0．05，P〈0．01）。碘化钾治疗3个月组FN蛋白表达为0．4068±0．0696，mRNA表达为0．7793±0．0613，与相应染铅组比较明显降低，差异有统计学意义（P〈0．05，P〈0．01）。结论碘化钾能改善染铅大鼠肾脏超微结构，可降低铅所导致的肾脏组织中核因子-κB及纤维连接蛋白的表达。Objective To investigate the effect of potassium iodide on the expression of nuclear factor-κB and fibronectin. Methods The experiment was performed with 72 SD rats weighing about 180-220 g. The animals were randomly assigned into nine groups. Group A, B, C （n=8） served as control and were fed with distilled water for 1 month, 2 month, 3 month respectively. Group D, E, F（n=8） served as lead exposed and were fed with water with 0.5% lead acetate for 1 month, 2 month, 3 month respectively. Group G, H, I （n=8） served as potassium iodide and lead exposed and were treated with 0.5% lead acetate simultaneously taking potassium iodide 3 mg/100 g weight by intragastric administration for 1 month, 2 month, 3 month respectively. Animals of different groups were sacrificed at the end of the treatment. Uhrastructure of kidney was observed by electron microscopy; Expression of NF-κB and FN protein and mRNA in kidney were measured respectively by immunohistochemistry and RT-PCR. Results Electron microscopic examination revealed potassium iodide could restrain the denaturalization in epithelial cells and mitochondrial cristae. The expressions of NF-κB protein （0.2315±0.0624,0.3213±0.0740,0.4729±0.0839） and mRNA （0.4370±0.0841,0.5465±0.0503,0.6443± 0.0538） in all the lead exposed groups continuously increased compared with correspondent control groups; Group I was decreased obviously compared with group F. The expressions of FN protein （0.4243 ± 0.0595,0.4917±0.0891 ） and mRNA （0.8650±0.0880,0.8714±0.0980） in group E and F increased compared with group B and C, but the expressions of FN protein in group I significantly decreased compared with group F; The expressions of FN mRNA in Group H and I significantly decreased compared with group E and F. Conclusion The potassium iodide can ameliorate renal uhrastrueture and degrade expression of nuclear factor-κB and fibronectin induced by lead.

关 键 词：碘化钾 铅 NF-ΚB 连接蛋白类 

分 类 号：R686[医药卫生—骨科学]