Pten敲除对Rad51基因表达的影响及其机制  

Effect of Pten Gene Knock-out on Expression of Rad51 Gene and Mechanism of The Effect

作　　者：莫琳[1,2] 陈忠明[2] 胡迎春[2] 米粲[1] 吴德昌[2] 霍艳英[2] 

机构地区：[1]重庆医科大学病理学教研室,重庆400016 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国生物制品学杂志》2009年第12期1179-1182,1186,共5页Chinese Journal of Biologicals

摘　　要：目的研究Pten敲除对Rad51基因表达的影响及其可能的机制。方法采用半定量RT-PCR法和克隆形成试验比较Pten+/+MEFs与Pten-/-MEFs两种细胞中同源重组修复相关基因Rad51mRNA转录水平的差异,并比较两种细胞对辐射敏感性的差异和辐射后Rad51基因mRNA转录水平的差异及其可能的机制。结果与Pten+/+MEFs细胞相比,Pten-/-MEFs细胞中Rad51基因mRNA的转录水平和对辐射的敏感性显著降低;辐射后以及PI3K/AKT信号通路抑制剂LY-294002作用后,Rad51基因mRNA的转录水平明显增高。结论Pten缺失后Rad51基因表达异常,Pten可能通过PI3K/AKT信号通路来调控Rad51基因的表达。Objective To investigate the effect of Pten gene knock-out on expression of Rad51 gene and the potential mechanism of the effect. Methods The mRNA transcription levels of Rad51 gene related to repair by homologous recombination in Pten＋/ ＋ MEFs and Pten-/ MEFs, the radiosensitivities of the two kinds of MEFs, as well as Rad51 mRNA transcription levels in the two kinds of MEFs after radiation, were compared by semi-quantitative RT-PCR and clone formation assay, and the potential mechanism was investigated. Results The Rad51 mRNA transcription level in Pten-/ MEFs was significantly lower than that in Pten＋ / ＋ MEFs. Compared with that of Pten＋ / ＋ MEFs, the radiosensitivity of Pten/ MEFs also decreased significantly. However, both the Rad51 mRNA transcription levels in Pten-/MEFs after radiation and after treatment with LY-294002, an inhibitor of P13K / AKT signal pathway, increased significantly. Conclusion Abnormal expression of Rad51 gene was observed after knock-out of Pten gene. Pten might regulate the expression of Rad51 gene through P13K / AKT signal pathway.

关 键 词：PTEN RAD51 基因敲除 表达 

分 类 号：Q789[生物学—分子生物学]